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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian UPF0059 membrane protein BT9727_5008 (BT9727_5008), partial CSB-YP750246BAAE1
CSB-EP750246BAAE1
CSB-BP750246BAAE1
CSB-MP750246BAAE1
CSB-EP750246BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian ATP synthase gamma chain (atpG) CSB-YP750247BAAE
CSB-EP750247BAAE
CSB-BP750247BAAE
CSB-MP750247BAAE
CSB-EP750247BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphomethylpyrimidine synthase (thiC), partial CSB-YP750248BAAE
CSB-EP750248BAAE
CSB-BP750248BAAE
CSB-MP750248BAAE
CSB-EP750248BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP750249BAAE1
CSB-EP750249BAAE1
CSB-BP750249BAAE1
CSB-MP750249BAAE1
CSB-EP750249BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian ATP-dependent Clp protease proteolytic subunit 2 (clpP2) CSB-YP750250BAAE
CSB-EP750250BAAE
CSB-BP750250BAAE
CSB-MP750250BAAE
CSB-EP750250BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Protein psiE homolog (psiE), partial CSB-YP750251BAAE1
CSB-EP750251BAAE1
CSB-BP750251BAAE1
CSB-MP750251BAAE1
CSB-EP750251BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Methionine import ATP-binding protein MetN 2 (metN2) CSB-YP750252BAAE
CSB-EP750252BAAE
CSB-BP750252BAAE
CSB-MP750252BAAE
CSB-EP750252BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable cytosol aminopeptidase (pepA) CSB-YP750253BAAE
CSB-EP750253BAAE
CSB-BP750253BAAE
CSB-MP750253BAAE
CSB-EP750253BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP750254BAAE
CSB-EP750254BAAE
CSB-BP750254BAAE
CSB-MP750254BAAE
CSB-EP750254BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0354 protein BT9727_4425 (BT9727_4425) CSB-YP750255BAAE
CSB-EP750255BAAE
CSB-BP750255BAAE
CSB-MP750255BAAE
CSB-EP750255BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable inorganic polyphosphate/ATP-NAD kinase 2 (ppnK2) CSB-YP750256BAAE
CSB-EP750256BAAE
CSB-BP750256BAAE
CSB-MP750256BAAE
CSB-EP750256BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP750257BAAE
CSB-EP750257BAAE
CSB-BP750257BAAE
CSB-MP750257BAAE
CSB-EP750257BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L35 (rpmI) CSB-YP750258BAAE
CSB-EP750258BAAE
CSB-BP750258BAAE
CSB-MP750258BAAE
CSB-EP750258BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Glutamate-1-semialdehyde 2,1-aminomutase 2 (hemL2) CSB-YP750259BAAE
CSB-EP750259BAAE
CSB-BP750259BAAE
CSB-MP750259BAAE
CSB-EP750259BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0758 protein BT9727_4187 (BT9727_4187) CSB-YP750260BAAE
CSB-EP750260BAAE
CSB-BP750260BAAE
CSB-MP750260BAAE
CSB-EP750260BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Quinolinate synthase A (nadA) CSB-YP750261BAAE
CSB-EP750261BAAE
CSB-BP750261BAAE
CSB-MP750261BAAE
CSB-EP750261BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Uncharacterized methyltransferase BT9727_4108 (BT9727_4108) CSB-YP750262BAAE
CSB-EP750262BAAE
CSB-BP750262BAAE
CSB-MP750262BAAE
CSB-EP750262BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S21 (rpsU) CSB-YP750263BAAE
CSB-EP750263BAAE
CSB-BP750263BAAE
CSB-MP750263BAAE
CSB-EP750263BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 3-dehydroquinate dehydratase (aroQ) CSB-YP750264BAAE
CSB-EP750264BAAE
CSB-BP750264BAAE
CSB-MP750264BAAE
CSB-EP750264BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP750265BAAE
CSB-EP750265BAAE
CSB-BP750265BAAE
CSB-MP750265BAAE
CSB-EP750265BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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