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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Glutamyl-tRNA reductase (hemA) CSB-YP750106SKV
CSB-EP750106SKV
CSB-BP750106SKV
CSB-MP750106SKV
CSB-EP750106SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0735 ACT domain-containing protein SAR1723 (SAR1723) CSB-YP750107SKV
CSB-EP750107SKV
CSB-BP750107SKV
CSB-MP750107SKV
CSB-EP750107SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0337 protein SAR1705 (SAR1705) CSB-YP750108SKV
CSB-EP750108SKV
CSB-BP750108SKV
CSB-MP750108SKV
CSB-EP750108SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Alanine--tRNA ligase (alaS), partial CSB-YP750109SKV
CSB-EP750109SKV
CSB-BP750109SKV
CSB-MP750109SKV
CSB-EP750109SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0473 protein SAR1694 (SAR1694) CSB-YP750110SKV
CSB-EP750110SKV
CSB-BP750110SKV
CSB-MP750110SKV
CSB-EP750110SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP750111SKV
CSB-EP750111SKV
CSB-BP750111SKV
CSB-MP750111SKV
CSB-EP750111SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTPase Era (era) CSB-YP750112SKV
CSB-EP750112SKV
CSB-BP750112SKV
CSB-MP750112SKV
CSB-EP750112SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0135 protein SAR1636 (SAR1636) CSB-YP750113SKV
CSB-EP750113SKV
CSB-BP750113SKV
CSB-MP750113SKV
CSB-EP750113SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ferric uptake regulation protein (fur) CSB-YP750114SKV
CSB-EP750114SKV
CSB-BP750114SKV
CSB-MP750114SKV
CSB-EP750114SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elongation factor P (efp) CSB-YP750115SKV
CSB-EP750115SKV
CSB-BP750115SKV
CSB-MP750115SKV
CSB-EP750115SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tyrosine recombinase XerD (xerD) CSB-YP750116SKV
CSB-EP750116SKV
CSB-BP750116SKV
CSB-MP750116SKV
CSB-EP750116SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S1 (rpsA) CSB-YP750117SKV
CSB-EP750117SKV
CSB-BP750117SKV
CSB-MP750117SKV
CSB-EP750117SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus CCA-adding enzyme (cca) CSB-YP750118SKV
CSB-EP750118SKV
CSB-BP750118SKV
CSB-MP750118SKV
CSB-EP750118SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Indole-3-glycerol phosphate synthase (trpC) CSB-YP750119SKV
CSB-EP750119SKV
CSB-BP750119SKV
CSB-MP750119SKV
CSB-EP750119SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide methionine sulfoxide reductase MsrA 1 (msrA1) CSB-YP750120SKV
CSB-EP750120SKV
CSB-BP750120SKV
CSB-MP750120SKV
CSB-EP750120SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Nuclease sbcCD subunit C (sbcC), partial CSB-YP750121SKV
CSB-EP750121SKV
CSB-BP750121SKV
CSB-MP750121SKV
CSB-EP750121SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GMP reductase (guaC) CSB-YP750122SKV
CSB-EP750122SKV
CSB-BP750122SKV
CSB-MP750122SKV
CSB-EP750122SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Homoserine kinase (thrB) CSB-YP750123SKV
CSB-EP750123SKV
CSB-BP750123SKV
CSB-MP750123SKV
CSB-EP750123SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol kinase (glpK) CSB-YP750124SKV
CSB-EP750124SKV
CSB-BP750124SKV
CSB-MP750124SKV
CSB-EP750124SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA pseudouridine synthase B (truB) CSB-YP750125SKV
CSB-EP750125SKV
CSB-BP750125SKV
CSB-MP750125SKV
CSB-EP750125SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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