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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP750266BAAE
CSB-EP750266BAAE
CSB-BP750266BAAE
CSB-MP750266BAAE
CSB-EP750266BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW) CSB-YP750267BAAE
CSB-EP750267BAAE
CSB-BP750267BAAE
CSB-MP750267BAAE
CSB-EP750267BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian N-acetyldiaminopimelate deacetylase (BT9727_3722) CSB-YP750268BAAE
CSB-EP750268BAAE
CSB-BP750268BAAE
CSB-MP750268BAAE
CSB-EP750268BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Heme A synthase (ctaA), partial CSB-YP750269BAAE
CSB-EP750269BAAE
CSB-BP750269BAAE
CSB-MP750269BAAE
CSB-EP750269BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP750270BAAE
CSB-EP750270BAAE
CSB-BP750270BAAE
CSB-MP750270BAAE
CSB-EP750270BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Orotate phosphoribosyltransferase (pyrE) CSB-YP750271BAAE
CSB-EP750271BAAE
CSB-BP750271BAAE
CSB-MP750271BAAE
CSB-EP750271BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L28 (rpmB) CSB-YP750272BAAE
CSB-EP750272BAAE
CSB-BP750272BAAE
CSB-MP750272BAAE
CSB-EP750272BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ribosome maturation factor RimM (rimM) CSB-YP750273BAAE
CSB-EP750273BAAE
CSB-BP750273BAAE
CSB-MP750273BAAE
CSB-EP750273BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP750274BAAE
CSB-EP750274BAAE
CSB-BP750274BAAE
CSB-MP750274BAAE
CSB-EP750274BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian tRNA pseudouridine synthase B (truB) CSB-YP750275BAAE
CSB-EP750275BAAE
CSB-BP750275BAAE
CSB-MP750275BAAE
CSB-EP750275BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Peptidase T (pepT) CSB-YP750276BAAE
CSB-EP750276BAAE
CSB-BP750276BAAE
CSB-MP750276BAAE
CSB-EP750276BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphonates import ATP-binding protein PhnC (phnC) CSB-YP750277BAAE
CSB-EP750277BAAE
CSB-BP750277BAAE
CSB-MP750277BAAE
CSB-EP750277BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0303 protein BT9727_3175 (BT9727_3175) CSB-YP750278BAAE
CSB-EP750278BAAE
CSB-BP750278BAAE
CSB-MP750278BAAE
CSB-EP750278BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Pyrrolidone-carboxylate peptidase (pcp) CSB-YP750279BAAE
CSB-EP750279BAAE
CSB-BP750279BAAE
CSB-MP750279BAAE
CSB-EP750279BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Kynureninase (kynU) CSB-YP750280BAAE
CSB-EP750280BAAE
CSB-BP750280BAAE
CSB-MP750280BAAE
CSB-EP750280BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Putative metal-dependent hydrolase BT9727_2476 (BT9727_2476) CSB-YP750281BAAE
CSB-EP750281BAAE
CSB-BP750281BAAE
CSB-MP750281BAAE
CSB-EP750281BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Methylmalonate semialdehyde dehydrogenase [acylating] 1 (iolA1) CSB-YP750282BAAE
CSB-EP750282BAAE
CSB-BP750282BAAE
CSB-MP750282BAAE
CSB-EP750282BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Arginine--tRNA ligase 1 (argS1), partial CSB-YP750283BAAE
CSB-EP750283BAAE
CSB-BP750283BAAE
CSB-MP750283BAAE
CSB-EP750283BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP750284BAAE
CSB-EP750284BAAE
CSB-BP750284BAAE
CSB-MP750284BAAE
CSB-EP750284BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Deoxyribose-phosphate aldolase (deoC) CSB-YP750285BAAE
CSB-EP750285BAAE
CSB-BP750285BAAE
CSB-MP750285BAAE
CSB-EP750285BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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