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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S17 (rpsQ) CSB-YP750306BAAE
CSB-EP750306BAAE
CSB-BP750306BAAE
CSB-MP750306BAAE
CSB-EP750306BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L1 (rplA) CSB-YP750307BAAE
CSB-EP750307BAAE
CSB-BP750307BAAE
CSB-MP750307BAAE
CSB-EP750307BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Lysine--tRNA ligase (lysS) CSB-YP750308BAAE
CSB-EP750308BAAE
CSB-BP750308BAAE
CSB-MP750308BAAE
CSB-EP750308BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0213 protein BT9727_0031 (BT9727_0031) CSB-YP750309BAAE
CSB-EP750309BAAE
CSB-BP750309BAAE
CSB-MP750309BAAE
CSB-EP750309BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP750310BAAE
CSB-EP750310BAAE
CSB-BP750310BAAE
CSB-MP750310BAAE
CSB-EP750310BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA pseudouridine synthase A 2 (truA2) CSB-YP750311BQE
CSB-EP750311BQE
CSB-BP750311BQE
CSB-MP750311BQE
CSB-EP750311BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cysteine proteinase inhibitor 3 (Os05g0409300, LOC_Os05g33880) CSB-YP750312OFG
CSB-EP750312OFG
CSB-BP750312OFG
CSB-MP750312OFG
CSB-EP750312OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica BURP domain-containing protein 2 (BURP2) CSB-YP750313OFG
CSB-EP750313OFG
CSB-BP750313OFG
CSB-MP750313OFG
CSB-EP750313OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Glutamine-dependent NAD (+) synthetase (NADSYN1) CSB-YP750314HU
CSB-EP750314HU
CSB-BP750314HU
CSB-MP750314HU
CSB-EP750314HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Postacrosomal sheath WW domain-binding protein (WBP2NL) CSB-YP750315HU
CSB-EP750315HU
CSB-BP750315HU
CSB-MP750315HU
CSB-EP750315HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Outer envelope pore protein 21A, chloroplastic (OEP21A), partial CSB-YP750316DOA
CSB-EP750316DOA
CSB-BP750316DOA
CSB-MP750316DOA
CSB-EP750316DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse OTU domain-containing protein 6A (Otud6a) CSB-YP750317MO
CSB-EP750317MO
CSB-BP750317MO
CSB-MP750317MO
CSB-EP750317MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ovostatin homolog 1 (OVOS1), partial CSB-YP750318HU
CSB-EP750318HU
CSB-BP750318HU
CSB-MP750318HU
CSB-EP750318HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serine protease inhibitor Kazal-type 7 (Spink7) CSB-YP750319RA
CSB-EP750319RA
CSB-BP750319RA
CSB-MP750319RA
CSB-EP750319RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Endonuclease 8-like 2 (NEIL2) CSB-YP750320BO
CSB-EP750320BO
CSB-BP750320BO
CSB-MP750320BO
CSB-EP750320BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human U11/U12 small nuclear ribonucleoprotein 48 kDa protein (SNRNP48) CSB-YP750321HU
CSB-EP750321HU
CSB-BP750321HU
CSB-MP750321HU
CSB-EP750321HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Olfactory receptor 7E24 (OR7E24), partial CSB-YP750322HU1
CSB-EP750322HU1
CSB-BP750322HU1
CSB-MP750322HU1
CSB-EP750322HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Keratin, type I cytoskeletal 42 (Krt42) CSB-YP750323RA
CSB-EP750323RA
CSB-BP750323RA
CSB-MP750323RA
CSB-EP750323RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Keratin, type I cytoskeletal 40 (Krt40) CSB-YP750324RA
CSB-EP750324RA
CSB-BP750324RA
CSB-MP750324RA
CSB-EP750324RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Keratin, type I cytoskeletal 27 (Krt27) CSB-YP750325RA
CSB-EP750325RA
CSB-BP750325RA
CSB-MP750325RA
CSB-EP750325RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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