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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Histidinol dehydrogenase (hisD) CSB-YP753036BHM
CSB-EP753036BHM
CSB-BP753036BHM
CSB-MP753036BHM
CSB-EP753036BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Disulfide bond formation protein B (dsbB), partial CSB-YP753037BHM1
CSB-EP753037BHM1
CSB-BP753037BHM1
CSB-MP753037BHM1
CSB-EP753037BHM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L32 (rpmF) CSB-YP753038BHM
CSB-EP753038BHM
CSB-BP753038BHM
CSB-MP753038BHM
CSB-EP753038BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine--tRNA ligase (serS) CSB-YP753039BHM
CSB-EP753039BHM
CSB-BP753039BHM
CSB-MP753039BHM
CSB-EP753039BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fumarate hydratase class II (fumC) CSB-YP753040BHM
CSB-EP753040BHM
CSB-BP753040BHM
CSB-MP753040BHM
CSB-EP753040BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP753041BHM
CSB-EP753041BHM
CSB-BP753041BHM
CSB-MP753041BHM
CSB-EP753041BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucine--tRNA ligase (leuS), partial CSB-YP753042BHM
CSB-EP753042BHM
CSB-BP753042BHM
CSB-MP753042BHM
CSB-EP753042BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP753043BHM
CSB-EP753043BHM
CSB-BP753043BHM
CSB-MP753043BHM
CSB-EP753043BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl pyrophosphate synthase (uppS) CSB-YP753044BHM
CSB-EP753044BHM
CSB-BP753044BHM
CSB-MP753044BHM
CSB-EP753044BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA pyrophosphohydrolase (rppH) CSB-YP753045BHM
CSB-EP753045BHM
CSB-BP753045BHM
CSB-MP753045BHM
CSB-EP753045BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP753046BHM
CSB-EP753046BHM
CSB-BP753046BHM
CSB-MP753046BHM
CSB-EP753046BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP753047BHM
CSB-EP753047BHM
CSB-BP753047BHM
CSB-MP753047BHM
CSB-EP753047BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L31 (rpmE) CSB-YP753048BHM
CSB-EP753048BHM
CSB-BP753048BHM
CSB-MP753048BHM
CSB-EP753048BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-dehydroquinate synthase (aroB) CSB-YP753049BHM
CSB-EP753049BHM
CSB-BP753049BHM
CSB-MP753049BHM
CSB-EP753049BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L10 (rplJ) CSB-YP753050BHM
CSB-EP753050BHM
CSB-BP753050BHM
CSB-MP753050BHM
CSB-EP753050BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP753051BHM
CSB-EP753051BHM
CSB-BP753051BHM
CSB-MP753051BHM
CSB-EP753051BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrodipicolinate synthase (dapA) CSB-YP753052BHM
CSB-EP753052BHM
CSB-BP753052BHM
CSB-MP753052BHM
CSB-EP753052BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chorismate synthase (aroC) CSB-YP753053BHM
CSB-EP753053BHM
CSB-BP753053BHM
CSB-MP753053BHM
CSB-EP753053BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP753054BHM
CSB-EP753054BHM
CSB-BP753054BHM
CSB-MP753054BHM
CSB-EP753054BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis DNA ligase (ligA), partial CSB-YP753055BUA
CSB-EP753055BUA
CSB-BP753055BUA
CSB-MP753055BUA
CSB-EP753055BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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