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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus 50S ribosomal protein L28 (rpmB) CSB-YP758479BQN
CSB-EP758479BQN
CSB-BP758479BQN
CSB-MP758479BQN
CSB-EP758479BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L19 (rplS) CSB-YP758480BQN
CSB-EP758480BQN
CSB-BP758480BQN
CSB-MP758480BQN
CSB-EP758480BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP758481BQN
CSB-EP758481BQN
CSB-BP758481BQN
CSB-MP758481BQN
CSB-EP758481BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Gag polyprotein (gag) CSB-YP758482HDAK
CSB-EP758482HDAK
CSB-BP758482HDAK
CSB-MP758482HDAK
CSB-EP758482HDAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Urease accessory protein UreE (ureE) CSB-YP758483BQN
CSB-EP758483BQN
CSB-BP758483BQN
CSB-MP758483BQN
CSB-EP758483BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein N (sspN) CSB-YP758484BQN
CSB-EP758484BQN
CSB-BP758484BQN
CSB-MP758484BQN
CSB-EP758484BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative regulator of ribonuclease activity (BCE_3327) CSB-YP758485BQN
CSB-EP758485BQN
CSB-BP758485BQN
CSB-MP758485BQN
CSB-EP758485BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chorismate synthase 2 (aroC2) CSB-YP758486BQN
CSB-EP758486BQN
CSB-BP758486BQN
CSB-MP758486BQN
CSB-EP758486BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Sec-independent protein translocase protein TatA (tatA), partial CSB-YP758487BQN
CSB-EP758487BQN
CSB-BP758487BQN
CSB-MP758487BQN
CSB-EP758487BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Deoxyribose-phosphate aldolase (deoC) CSB-YP758488BQN
CSB-EP758488BQN
CSB-BP758488BQN
CSB-MP758488BQN
CSB-EP758488BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus CCA-adding enzyme (cca) CSB-YP758489BQN
CSB-EP758489BQN
CSB-BP758489BQN
CSB-MP758489BQN
CSB-EP758489BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chorismate synthase 1 (aroC1) CSB-YP758490BQN
CSB-EP758490BQN
CSB-BP758490BQN
CSB-MP758490BQN
CSB-EP758490BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ketol-acid reductoisomerase 1 (ilvC1) CSB-YP758491BQN
CSB-EP758491BQN
CSB-BP758491BQN
CSB-MP758491BQN
CSB-EP758491BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bis (5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] (prpE) CSB-YP758492BQN
CSB-EP758492BQN
CSB-BP758492BQN
CSB-MP758492BQN
CSB-EP758492BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chaperone protein ClpB (clpB), partial CSB-YP758493BQN
CSB-EP758493BQN
CSB-BP758493BQN
CSB-MP758493BQN
CSB-EP758493BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0145 protein BCE_1095 (BCE_1095) CSB-YP758494BQN
CSB-EP758494BQN
CSB-BP758494BQN
CSB-MP758494BQN
CSB-EP758494BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Quinol oxidase subunit 2 (qoxA), partial CSB-YP758495BQN1
CSB-EP758495BQN1
CSB-BP758495BQN1
CSB-MP758495BQN1
CSB-EP758495BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0374 protein BCE_0582 (BCE_0582) CSB-YP758496BQN
CSB-EP758496BQN
CSB-BP758496BQN
CSB-MP758496BQN
CSB-EP758496BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA ligase (ligA), partial CSB-YP758497BQN
CSB-EP758497BQN
CSB-BP758497BQN
CSB-MP758497BQN
CSB-EP758497BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Redox-sensing transcriptional repressor rex (rex) CSB-YP758498BQN
CSB-EP758498BQN
CSB-BP758498BQN
CSB-MP758498BQN
CSB-EP758498BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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