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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria monocytogenes serotype 4b 30S ribosomal protein S7 (rpsG) CSB-YP758278LAAD
CSB-EP758278LAAD
CSB-BP758278LAAD
CSB-MP758278LAAD
CSB-EP758278LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L5 (rplE) CSB-YP758279LAAD
CSB-EP758279LAAD
CSB-BP758279LAAD
CSB-MP758279LAAD
CSB-EP758279LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Translation initiation factor IF-1 (infA) CSB-YP758280LAAD
CSB-EP758280LAAD
CSB-BP758280LAAD
CSB-MP758280LAAD
CSB-EP758280LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 30S ribosomal protein S13 (rpsM) CSB-YP758281LAAD
CSB-EP758281LAAD
CSB-BP758281LAAD
CSB-MP758281LAAD
CSB-EP758281LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Energy-coupling factor transporter ATP-binding protein EcfA 1 (ecfA1) CSB-YP758282LAAD
CSB-EP758282LAAD
CSB-BP758282LAAD
CSB-MP758282LAAD
CSB-EP758282LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Probable tautomerase LMOf2365_2536 (LMOf2365_2536) CSB-YP758283LAAD
CSB-EP758283LAAD
CSB-BP758283LAAD
CSB-MP758283LAAD
CSB-EP758283LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Peptide chain release factor 1 (prfA) CSB-YP758284LAAD
CSB-EP758284LAAD
CSB-BP758284LAAD
CSB-MP758284LAAD
CSB-EP758284LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b ATP synthase subunit b (atpF), partial CSB-YP758285LAAD1
CSB-EP758285LAAD1
CSB-BP758285LAAD1
CSB-MP758285LAAD1
CSB-EP758285LAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Protein translocase subunit SecA 1 (secA1), partial CSB-YP758286LAAD
CSB-EP758286LAAD
CSB-BP758286LAAD
CSB-MP758286LAAD
CSB-EP758286LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative sporulation transcription regulator WhiA (whiA) CSB-YP758287LAAD
CSB-EP758287LAAD
CSB-BP758287LAAD
CSB-MP758287LAAD
CSB-EP758287LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP758288LAAD
CSB-EP758288LAAD
CSB-BP758288LAAD
CSB-MP758288LAAD
CSB-EP758288LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0344 protein LMOf2365_2298 (LMOf2365_2298), partial CSB-YP758289LAAD1
CSB-EP758289LAAD1
CSB-BP758289LAAD1
CSB-MP758289LAAD1
CSB-EP758289LAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Chaperone protein ClpB (clpB), partial CSB-YP758290LAAD
CSB-EP758290LAAD
CSB-BP758290LAAD
CSB-MP758290LAAD
CSB-EP758290LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP758291LAAD
CSB-EP758291LAAD
CSB-BP758291LAAD
CSB-MP758291LAAD
CSB-EP758291LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Protoheme IX farnesyltransferase (ctaB), partial CSB-YP758292LAAD
CSB-EP758292LAAD
CSB-BP758292LAAD
CSB-MP758292LAAD
CSB-EP758292LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP758293LAAD
CSB-EP758293LAAD
CSB-BP758293LAAD
CSB-MP758293LAAD
CSB-EP758293LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP758294LAAD
CSB-EP758294LAAD
CSB-BP758294LAAD
CSB-MP758294LAAD
CSB-EP758294LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP758295LAAD
CSB-EP758295LAAD
CSB-BP758295LAAD
CSB-MP758295LAAD
CSB-EP758295LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b DNA polymerase IV (dinB) CSB-YP758296LAAD
CSB-EP758296LAAD
CSB-BP758296LAAD
CSB-MP758296LAAD
CSB-EP758296LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Segregation and condensation protein B (scpB) CSB-YP758297LAAD
CSB-EP758297LAAD
CSB-BP758297LAAD
CSB-MP758297LAAD
CSB-EP758297LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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