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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Petunia vein clearing virus Genome polyprotein, partial CSB-YP757847PHAT
CSB-EP757847PHAT
CSB-BP757847PHAT
CSB-MP757847PHAT
CSB-EP757847PHAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opistophthalmus carinatus Potassium channel toxin alpha-KTx 6.7 CSB-YP757848OBW
CSB-EP757848OBW
CSB-BP757848OBW
CSB-MP757848OBW
CSB-EP757848OBW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Encephalitozoon hellem Methionine aminopeptidase 2 (MAP2) CSB-YP757849EKI
CSB-EP757849EKI
CSB-BP757849EKI
CSB-MP757849EKI
CSB-EP757849EKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase TPTE2 (TPTE2), partial CSB-YP757850HU1
CSB-EP757850HU1
CSB-BP757850HU1
CSB-MP757850HU1
CSB-EP757850HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Carbohydrate sulfotransferase 7 (Chst7), partial CSB-YP757851RA
CSB-EP757851RA
CSB-BP757851RA
CSB-MP757851RA
CSB-EP757851RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allpahuayo virus RNA-directed RNA polymerase L (L), partial CSB-YP757852AZR
CSB-EP757852AZR
CSB-BP757852AZR
CSB-MP757852AZR
CSB-EP757852AZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP757853IDG
CSB-EP757853IDG
CSB-BP757853IDG
CSB-MP757853IDG
CSB-EP757853IDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase acidic protein (PA), partial CSB-YP757854IGA
CSB-EP757854IGA
CSB-BP757854IGA
CSB-MP757854IGA
CSB-EP757854IGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 1 (M) CSB-YP757855IEV
CSB-EP757855IEV
CSB-BP757855IEV
CSB-MP757855IEV
CSB-EP757855IEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plecoglossus altivelis Metallothionein (mt) CSB-YP757856PHAU
CSB-EP757856PHAU
CSB-BP757856PHAU
CSB-MP757856PHAU
CSB-EP757856PHAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Neuraminidase (NA), partial CSB-YP757857IEI
CSB-EP757857IEI
CSB-BP757857IEI
CSB-MP757857IEI
CSB-EP757857IEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog 5-hydroxytryptamine receptor 1A (HTR1A), partial CSB-YP757858DO1
CSB-EP757858DO1
CSB-BP757858DO1
CSB-MP757858DO1
CSB-EP757858DO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transcription factor LBX2 (LBX2) CSB-YP757859HU
CSB-EP757859HU
CSB-BP757859HU
CSB-MP757859HU
CSB-EP757859HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L6 (rplF) CSB-YP757860SJV
CSB-EP757860SJV
CSB-BP757860SJV
CSB-MP757860SJV
CSB-EP757860SJV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L22 (rplV) CSB-YP757861SJV
CSB-EP757861SJV
CSB-BP757861SJV
CSB-MP757861SJV
CSB-EP757861SJV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Dynamin-binding protein (DNMBP), partial CSB-YP757862HU
CSB-EP757862HU
CSB-BP757862HU
CSB-MP757862HU
CSB-EP757862HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyvera citrophila DNA protection during starvation protein (dps) CSB-YP757863KAL
CSB-EP757863KAL
CSB-BP757863KAL
CSB-MP757863KAL
CSB-EP757863KAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA protection during starvation protein (dps) CSB-YP757864PSP
CSB-EP757864PSP
CSB-BP757864PSP
CSB-MP757864PSP
CSB-EP757864PSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pagrus major Ragulator complex protein LAMTOR3 (lamtor3) CSB-YP757865EQR
CSB-EP757865EQR
CSB-BP757865EQR
CSB-MP757865EQR
CSB-EP757865EQR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Rho GTPase-activating protein 10 (Arhgap10) CSB-YP757866MO
CSB-EP757866MO
CSB-BP757866MO
CSB-MP757866MO
CSB-EP757866MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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