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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Germin-like protein 8-10 (GLP2) CSB-YP757907OFG
CSB-EP757907OFG
CSB-BP757907OFG
CSB-MP757907OFG
CSB-EP757907OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY (SPY), partial CSB-YP757908OFG
CSB-EP757908OFG
CSB-BP757908OFG
CSB-MP757908OFG
CSB-EP757908OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Germin-like protein 8-3 (GER2) CSB-YP757909OFG
CSB-EP757909OFG
CSB-BP757909OFG
CSB-MP757909OFG
CSB-EP757909OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica CASP-like protein Os08g0536200 (Os08g0536200, LOC_Os08g42430), partial CSB-YP757910OFG1
CSB-EP757910OFG1
CSB-BP757910OFG1
CSB-MP757910OFG1
CSB-EP757910OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable calcium-binding protein CML20 (CML20) CSB-YP757911OFG
CSB-EP757911OFG
CSB-BP757911OFG
CSB-MP757911OFG
CSB-EP757911OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Geranylgeranyl diphosphate reductase, chloroplastic (CHLP) CSB-YP757912OFG
CSB-EP757912OFG
CSB-BP757912OFG
CSB-MP757912OFG
CSB-EP757912OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Zinc finger CCCH domain-containing protein 49 (Os07g0281000, LOC_Os07g18050) CSB-YP757913OFG
CSB-EP757913OFG
CSB-BP757913OFG
CSB-MP757913OFG
CSB-EP757913OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Clathrin light chain 3 (Os07g0461500, LOC_Os07g27750) CSB-YP757914OFG
CSB-EP757914OFG
CSB-BP757914OFG
CSB-MP757914OFG
CSB-EP757914OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica UDP-arabinopyranose mutase 3 (UAM3) CSB-YP757915OFG
CSB-EP757915OFG
CSB-BP757915OFG
CSB-MP757915OFG
CSB-EP757915OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Thioredoxin-like 1-1, chloroplastic (Os07g0684100, LOC_Os07g48510) CSB-YP757916OFG
CSB-EP757916OFG
CSB-BP757916OFG
CSB-MP757916OFG
CSB-EP757916OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Lysine-specific histone demethylase 1 homolog 1 (Os02g0755200, LOC_Os02g51880), partial CSB-YP757917OFG
CSB-EP757917OFG
CSB-BP757917OFG
CSB-MP757917OFG
CSB-EP757917OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica MADS-box transcription factor 57 (MADS57) CSB-YP757918OFG
CSB-EP757918OFG
CSB-BP757918OFG
CSB-MP757918OFG
CSB-EP757918OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Endoglucanase 4 (GLU14) CSB-YP757919OFG
CSB-EP757919OFG
CSB-BP757919OFG
CSB-MP757919OFG
CSB-EP757919OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Metal tolerance protein 3 (MTP3), partial CSB-YP757920OFG1
CSB-EP757920OFG1
CSB-BP757920OFG1
CSB-MP757920OFG1
CSB-EP757920OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Squamosa promoter-binding-like protein 15 (SPL15), partial CSB-YP757921OFG
CSB-EP757921OFG
CSB-BP757921OFG
CSB-MP757921OFG
CSB-EP757921OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Heat stress transcription factor B-2b (HSFB2B) CSB-YP757922OFG
CSB-EP757922OFG
CSB-BP757922OFG
CSB-MP757922OFG
CSB-EP757922OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable 4-coumarate--CoA ligase 5 (4CL5), partial CSB-YP757923OFG
CSB-EP757923OFG
CSB-BP757923OFG
CSB-MP757923OFG
CSB-EP757923OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica bZIP transcription factor TRAB1 (TRAB1) CSB-YP757924OFG
CSB-EP757924OFG
CSB-BP757924OFG
CSB-MP757924OFG
CSB-EP757924OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable mixed-linked glucan synthase 3 (CSLF3), partial CSB-YP757925OFG
CSB-EP757925OFG
CSB-BP757925OFG
CSB-MP757925OFG
CSB-EP757925OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Rac-like GTP-binding protein 6 (RAC6) CSB-YP757926OFG
CSB-EP757926OFG
CSB-BP757926OFG
CSB-MP757926OFG
CSB-EP757926OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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