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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Desulfovibrio vulgaris Aspartate--tRNA ligase (aspS), partial CSB-YP758339DDH
CSB-EP758339DDH
CSB-BP758339DDH
CSB-MP758339DDH
CSB-EP758339DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris UPF0173 metal-dependent hydrolase DVU_3308 (DVU_3308) CSB-YP758340DDH
CSB-EP758340DDH
CSB-BP758340DDH
CSB-MP758340DDH
CSB-EP758340DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP758341DDH
CSB-EP758341DDH
CSB-BP758341DDH
CSB-MP758341DDH
CSB-EP758341DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 50S ribosomal protein L10 (rplJ) CSB-YP758342DDH
CSB-EP758342DDH
CSB-BP758342DDH
CSB-MP758342DDH
CSB-EP758342DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP758343DDH
CSB-EP758343DDH
CSB-BP758343DDH
CSB-MP758343DDH
CSB-EP758343DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Bifunctional protein GlmU (glmU) CSB-YP758344DDH
CSB-EP758344DDH
CSB-BP758344DDH
CSB-MP758344DDH
CSB-EP758344DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP758345DDH
CSB-EP758345DDH
CSB-BP758345DDH
CSB-MP758345DDH
CSB-EP758345DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP758346DDH
CSB-EP758346DDH
CSB-BP758346DDH
CSB-MP758346DDH
CSB-EP758346DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP758347DDH
CSB-EP758347DDH
CSB-BP758347DDH
CSB-MP758347DDH
CSB-EP758347DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Thymidylate synthase thyX (thyX) CSB-YP758348DDH
CSB-EP758348DDH
CSB-BP758348DDH
CSB-MP758348DDH
CSB-EP758348DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP758349DDH
CSB-EP758349DDH
CSB-BP758349DDH
CSB-MP758349DDH
CSB-EP758349DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP758350DDH
CSB-EP758350DDH
CSB-BP758350DDH
CSB-MP758350DDH
CSB-EP758350DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 30S ribosomal protein S21 (rpsU) CSB-YP758351DDH
CSB-EP758351DDH
CSB-BP758351DDH
CSB-MP758351DDH
CSB-EP758351DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP758352DDH
CSB-EP758352DDH
CSB-BP758352DDH
CSB-MP758352DDH
CSB-EP758352DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 50S ribosomal protein L25 (rplY) CSB-YP758353DDH
CSB-EP758353DDH
CSB-BP758353DDH
CSB-MP758353DDH
CSB-EP758353DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Phosphopantetheine adenylyltransferase (coaD) CSB-YP758354DDH
CSB-EP758354DDH
CSB-BP758354DDH
CSB-MP758354DDH
CSB-EP758354DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP758355DDH
CSB-EP758355DDH
CSB-BP758355DDH
CSB-MP758355DDH
CSB-EP758355DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 50S ribosomal protein L17 (rplQ) CSB-YP758356DDH
CSB-EP758356DDH
CSB-BP758356DDH
CSB-MP758356DDH
CSB-EP758356DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 30S ribosomal protein S14 type Z (rpsZ) CSB-YP758357DDH
CSB-EP758357DDH
CSB-BP758357DDH
CSB-MP758357DDH
CSB-EP758357DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 50S ribosomal protein L23 (rplW) CSB-YP758358DDH
CSB-EP758358DDH
CSB-BP758358DDH
CSB-MP758358DDH
CSB-EP758358DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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