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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Peroxide-responsive repressor perR (perR) CSB-YP756865SKV
CSB-EP756865SKV
CSB-BP756865SKV
CSB-MP756865SKV
CSB-EP756865SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP756866SKV
CSB-EP756866SKV
CSB-BP756866SKV
CSB-MP756866SKV
CSB-EP756866SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein CrcB homolog 1 (crcB1), partial CSB-YP756867SKV1
CSB-EP756867SKV1
CSB-BP756867SKV1
CSB-MP756867SKV1
CSB-EP756867SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP756868SKV
CSB-EP756868SKV
CSB-BP756868SKV
CSB-MP756868SKV
CSB-EP756868SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP756869SKV
CSB-EP756869SKV
CSB-BP756869SKV
CSB-MP756869SKV
CSB-EP756869SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Alanine dehydrogenase 2 (ald2) CSB-YP756870SKV
CSB-EP756870SKV
CSB-BP756870SKV
CSB-MP756870SKV
CSB-EP756870SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 6-phosphofructokinase (pfkA) CSB-YP756871SKV
CSB-EP756871SKV
CSB-BP756871SKV
CSB-MP756871SKV
CSB-EP756871SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Isocitrate dehydrogenase [NADP] (icd) CSB-YP756872SKV
CSB-EP756872SKV
CSB-BP756872SKV
CSB-MP756872SKV
CSB-EP756872SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Porphobilinogen deaminase (hemC) CSB-YP756873SKV
CSB-EP756873SKV
CSB-BP756873SKV
CSB-MP756873SKV
CSB-EP756873SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP756874SKV
CSB-EP756874SKV
CSB-BP756874SKV
CSB-MP756874SKV
CSB-EP756874SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0297 protein SAR1696 (SAR1696) CSB-YP756875SKV
CSB-EP756875SKV
CSB-BP756875SKV
CSB-MP756875SKV
CSB-EP756875SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Shikimate dehydrogenase (aroE) CSB-YP756876SKV
CSB-EP756876SKV
CSB-BP756876SKV
CSB-MP756876SKV
CSB-EP756876SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chaperone protein DnaJ (dnaJ) CSB-YP756877SKV
CSB-EP756877SKV
CSB-BP756877SKV
CSB-MP756877SKV
CSB-EP756877SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative phosphotransferase SAR1640 (SAR1640) CSB-YP756878SKV
CSB-EP756878SKV
CSB-BP756878SKV
CSB-MP756878SKV
CSB-EP756878SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP756879SKV
CSB-EP756879SKV
CSB-BP756879SKV
CSB-MP756879SKV
CSB-EP756879SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Segregation and condensation protein B (scpB) CSB-YP756880SKV
CSB-EP756880SKV
CSB-BP756880SKV
CSB-MP756880SKV
CSB-EP756880SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cytidylate kinase (cmk) CSB-YP756881SKV
CSB-EP756881SKV
CSB-BP756881SKV
CSB-MP756881SKV
CSB-EP756881SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA-binding protein HU (hup) CSB-YP756882SKV
CSB-EP756882SKV
CSB-BP756882SKV
CSB-MP756882SKV
CSB-EP756882SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0302 protein SAR1473 (SAR1473) CSB-YP756883SKV
CSB-EP756883SKV
CSB-BP756883SKV
CSB-MP756883SKV
CSB-EP756883SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cell cycle protein GpsB (gpsB) CSB-YP756884SKV
CSB-EP756884SKV
CSB-BP756884SKV
CSB-MP756884SKV
CSB-EP756884SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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