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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii Cytochrome oxidase assembly protein 3, mitochondrial (COA3), partial CSB-YP758739DOT1
CSB-EP758739DOT1
CSB-BP758739DOT1
CSB-MP758739DOT1
CSB-EP758739DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ras modification protein ERF4 (ERF4) CSB-YP758740DOT
CSB-EP758740DOT
CSB-BP758740DOT
CSB-MP758740DOT
CSB-EP758740DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP synthase subunit 4, mitochondrial (ATP4) CSB-YP758741DOT
CSB-EP758741DOT
CSB-BP758741DOT
CSB-MP758741DOT
CSB-EP758741DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DASH complex subunit SPC34 (SPC34) CSB-YP758742DOT
CSB-EP758742DOT
CSB-BP758742DOT
CSB-MP758742DOT
CSB-EP758742DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii mRNA 3'-end-processing protein YTH1 (YTH1) CSB-YP758743DOT
CSB-EP758743DOT
CSB-BP758743DOT
CSB-MP758743DOT
CSB-EP758743DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial Rho GTPase 1 (GEM1), partial CSB-YP758744DOT
CSB-EP758744DOT
CSB-BP758744DOT
CSB-MP758744DOT
CSB-EP758744DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii AP-3 complex subunit beta (APL6), partial CSB-YP758745DOT
CSB-EP758745DOT
CSB-BP758745DOT
CSB-MP758745DOT
CSB-EP758745DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Outer spore wall protein 5 (OSW5), partial CSB-YP758746DOT
CSB-EP758746DOT
CSB-BP758746DOT
CSB-MP758746DOT
CSB-EP758746DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 60S ribosomal protein L3 (RPL3) CSB-YP758747DOT
CSB-EP758747DOT
CSB-BP758747DOT
CSB-MP758747DOT
CSB-EP758747DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Endoplasmic reticulum vesicle protein 25 (ERV25), partial CSB-YP758748DOT
CSB-EP758748DOT
CSB-BP758748DOT
CSB-MP758748DOT
CSB-EP758748DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial import inner membrane translocase subunit TIM10 (TIM10) CSB-YP758749DOT
CSB-EP758749DOT
CSB-BP758749DOT
CSB-MP758749DOT
CSB-EP758749DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii tRNA-splicing endonuclease subunit SEN15 (SEN15) CSB-YP758750DOT
CSB-EP758750DOT
CSB-BP758750DOT
CSB-MP758750DOT
CSB-EP758750DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DNA-directed RNA polymerase II subunit RPB1 (RPB1), partial CSB-YP758751DOT
CSB-EP758751DOT
CSB-BP758751DOT
CSB-MP758751DOT
CSB-EP758751DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Antagonist of mitotic exit network protein 1 (AMN1) CSB-YP758752DOT
CSB-EP758752DOT
CSB-BP758752DOT
CSB-MP758752DOT
CSB-EP758752DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Multiple RNA-binding domain-containing protein 1 (MRD1), partial CSB-YP758753DOT
CSB-EP758753DOT
CSB-BP758753DOT
CSB-MP758753DOT
CSB-EP758753DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Nucleolar protein 16 (NOP16) CSB-YP758754DOT
CSB-EP758754DOT
CSB-BP758754DOT
CSB-MP758754DOT
CSB-EP758754DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Golgin IMH1 (IMH1), partial CSB-YP758755DOT
CSB-EP758755DOT
CSB-BP758755DOT
CSB-MP758755DOT
CSB-EP758755DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Respiratory growth induced protein 1 (RGI1) CSB-YP758756DOT
CSB-EP758756DOT
CSB-BP758756DOT
CSB-MP758756DOT
CSB-EP758756DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Regulator of rDNA transcription protein 5 (RRT5) CSB-YP758757DOT
CSB-EP758757DOT
CSB-BP758757DOT
CSB-MP758757DOT
CSB-EP758757DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Peroxisomal membrane protein PEX21 (PEX21) CSB-YP758758DOT
CSB-EP758758DOT
CSB-BP758758DOT
CSB-MP758758DOT
CSB-EP758758DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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