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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aspergillus niger Eukaryotic translation initiation factor 3 subunit D (An15g03780), partial CSB-YP007533AVE
CSB-EP007533AVE
CSB-BP007533AVE
CSB-MP007533AVE
CSB-EP007533AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Peptide chain release factor 1 (prfA) CSB-YP015197CWT
CSB-EP015197CWT
CSB-BP015197CWT
CSB-MP015197CWT
CSB-EP015197CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Lipoyl synthase (lipA) CSB-YP012927CWT
CSB-EP012927CWT
CSB-BP012927CWT
CSB-MP012927CWT
CSB-EP012927CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Elongation factor Ts (tsf) CSB-YP025124CWT
CSB-EP025124CWT
CSB-BP025124CWT
CSB-MP025124CWT
CSB-EP025124CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Argininosuccinate synthase (argG) CSB-YP002234CWT
CSB-EP002234CWT
CSB-BP002234CWT
CSB-MP002234CWT
CSB-EP002234CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Translation initiation factor IF-2 (infB), partial CSB-YP015164CWT
CSB-EP015164CWT
CSB-BP015164CWT
CSB-MP015164CWT
CSB-EP015164CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Elongation factor G (fusA), partial CSB-YP009373CWT
CSB-EP009373CWT
CSB-BP009373CWT
CSB-MP009373CWT
CSB-EP009373CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii Elongation factor Tu (tuf1) CSB-YP025342VDY
CSB-EP025342VDY
CSB-BP025342VDY
CSB-MP025342VDY
CSB-EP025342VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii Cysteine desulfurase (iscS) CSB-YP015769VDY
CSB-EP015769VDY
CSB-BP015769VDY
CSB-MP015769VDY
CSB-EP015769VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii Thymidylate kinase (tmk) CSB-YP007226VDY
CSB-EP007226VDY
CSB-BP007226VDY
CSB-MP007226VDY
CSB-EP007226VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii Protoheme IX farnesyltransferase (cyoE), partial CSB-YP005824VDY
CSB-EP005824VDY
CSB-BP005824VDY
CSB-MP005824VDY
CSB-EP005824VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146PYG
CSB-EP019146PYG
CSB-BP019146PYG
CSB-MP019146PYG
CSB-EP019146PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Argininosuccinate synthase (argG) CSB-YP002234PYG
CSB-EP002234PYG
CSB-BP002234PYG
CSB-MP002234PYG
CSB-EP002234PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Cytoplasmic tRNA 2-thiolation protein 2 (NCS2) CSB-YP003313MTM
CSB-EP003313MTM
CSB-BP003313MTM
CSB-MP003313MTM
CSB-EP003313MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Ubiquitin-like modifier-activating enzyme ATG7 (ATG7), partial CSB-YP002294MTM
CSB-EP002294MTM
CSB-BP002294MTM
CSB-MP002294MTM
CSB-EP002294MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Exportin-T (LOS1), partial CSB-YP026226MTM
CSB-EP026226MTM
CSB-BP026226MTM
CSB-MP026226MTM
CSB-EP026226MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Eukaryotic translation initiation factor 3 subunit G (TIF35) CSB-YP007536MTM
CSB-EP007536MTM
CSB-BP007536MTM
CSB-MP007536MTM
CSB-EP007536MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii NADH-cytochrome b5 reductase 1 (CBR1), partial CSB-YP006318MTM1
CSB-EP006318MTM1
CSB-BP006318MTM1
CSB-MP006318MTM1
CSB-EP006318MTM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii NADH-cytochrome b5 reductase 2 (MCR1), partial CSB-YP006319MTM1
CSB-EP006319MTM1
CSB-BP006319MTM1
CSB-MP006319MTM1
CSB-EP006319MTM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Cytoplasmic tRNA 2-thiolation protein 2 (NCS2) CSB-YP003313LLV
CSB-EP003313LLV
CSB-BP003313LLV
CSB-MP003313LLV
CSB-EP003313LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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