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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bovine TRMT1-like protein (TRMT1L), partial CSB-YP003588BO
CSB-EP003588BO
CSB-BP003588BO
CSB-MP003588BO
CSB-EP003588BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Autophagy protein 5 (ATG5) CSB-YP002293MTM
CSB-EP002293MTM
CSB-BP002293MTM
CSB-MP002293MTM
CSB-EP002293MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Ribose-5-phosphate isomerase (RKI1) CSB-YP020104MTM
CSB-EP020104MTM
CSB-BP020104MTM
CSB-MP020104MTM
CSB-EP020104MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Pescadillo homolog (NOP7), partial CSB-YP017791MTM
CSB-EP017791MTM
CSB-BP017791MTM
CSB-MP017791MTM
CSB-EP017791MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Eukaryotic translation initiation factor 3 subunit A (TIF32), partial CSB-YP007543MTM
CSB-EP007543MTM
CSB-BP007543MTM
CSB-MP007543MTM
CSB-EP007543MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Eukaryotic translation initiation factor 3 subunit J (HCR1) CSB-YP007539MTM
CSB-EP007539MTM
CSB-BP007539MTM
CSB-MP007539MTM
CSB-EP007539MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Histone H2A.Z (HTZ1) CSB-YP010100MTM
CSB-EP010100MTM
CSB-BP010100MTM
CSB-MP010100MTM
CSB-EP010100MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus DNA-directed RNA polymerase III subunit RPC3 (RPC82), partial CSB-YP018345LLV
CSB-EP018345LLV
CSB-BP018345LLV
CSB-MP018345LLV
CSB-EP018345LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus mRNA cap guanine-N7 methyltransferase (ABD1), partial CSB-YP019902LLV
CSB-EP019902LLV
CSB-BP019902LLV
CSB-MP019902LLV
CSB-EP019902LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Flap endonuclease 1 (FEN1) CSB-YP008585LLV
CSB-EP008585LLV
CSB-BP008585LLV
CSB-MP008585LLV
CSB-EP008585LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Eukaryotic translation initiation factor 3 subunit J (HCR1) CSB-YP007539LLV
CSB-EP007539LLV
CSB-BP007539LLV
CSB-MP007539LLV
CSB-EP007539LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Exportin-T (LOS1), partial CSB-YP026226LLV
CSB-EP026226LLV
CSB-BP026226LLV
CSB-MP026226LLV
CSB-EP026226LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Argininosuccinate synthase (argG) CSB-YP002234BPE
CSB-EP002234BPE
CSB-BP002234BPE
CSB-MP002234BPE
CSB-EP002234BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451BPE
CSB-EP015451BPE
CSB-BP015451BPE
CSB-MP015451BPE
CSB-EP015451BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Malate dehydrogenase (mdh) CSB-YP013623BPE
CSB-EP013623BPE
CSB-BP013623BPE
CSB-MP013623BPE
CSB-EP013623BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. ATP synthase subunit b (atpF), partial CSB-YP002358BPE1
CSB-EP002358BPE1
CSB-BP002358BPE1
CSB-MP002358BPE1
CSB-EP002358BPE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Elongation factor G (fusA), partial CSB-YP009373BPE
CSB-EP009373BPE
CSB-BP009373BPE
CSB-MP009373BPE
CSB-EP009373BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. tRNA dimethylallyltransferase (miaA) CSB-YP024529BPE
CSB-EP024529BPE
CSB-BP024529BPE
CSB-MP024529BPE
CSB-EP024529BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Urocanate hydratase (hutU), partial CSB-YP025677BPE
CSB-EP025677BPE
CSB-BP025677BPE
CSB-MP025677BPE
CSB-EP025677BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Phosphoserine aminotransferase (serC) CSB-YP018838DHZ
CSB-EP018838DHZ
CSB-BP018838DHZ
CSB-MP018838DHZ
CSB-EP018838DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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