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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermus thermophilus Glucose-6-phosphate isomerase (pgi) CSB-YP758379TNS
CSB-EP758379TNS
CSB-BP758379TNS
CSB-MP758379TNS
CSB-EP758379TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Queuine tRNA-ribosyltransferase (tgt) CSB-YP758380TNS
CSB-EP758380TNS
CSB-BP758380TNS
CSB-MP758380TNS
CSB-EP758380TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 6-phosphofructokinase (pfkA) CSB-YP758381TNS
CSB-EP758381TNS
CSB-BP758381TNS
CSB-MP758381TNS
CSB-EP758381TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Putative Holliday junction resolvase (TT_C1478) CSB-YP758382TNS
CSB-EP758382TNS
CSB-BP758382TNS
CSB-MP758382TNS
CSB-EP758382TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L27 (rpmA) CSB-YP758383TNS
CSB-EP758383TNS
CSB-BP758383TNS
CSB-MP758383TNS
CSB-EP758383TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L4 (rplD) CSB-YP758384TNS
CSB-EP758384TNS
CSB-BP758384TNS
CSB-MP758384TNS
CSB-EP758384TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L16 (rplP) CSB-YP758385TNS
CSB-EP758385TNS
CSB-BP758385TNS
CSB-MP758385TNS
CSB-EP758385TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP758386TNS
CSB-EP758386TNS
CSB-BP758386TNS
CSB-MP758386TNS
CSB-EP758386TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Recombination protein RecR (recR) CSB-YP758387TNS
CSB-EP758387TNS
CSB-BP758387TNS
CSB-MP758387TNS
CSB-EP758387TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Peptidyl-tRNA hydrolase (pth) CSB-YP758388TNS
CSB-EP758388TNS
CSB-BP758388TNS
CSB-MP758388TNS
CSB-EP758388TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus S-ribosylhomocysteine lyase (luxS) CSB-YP758389TNS
CSB-EP758389TNS
CSB-BP758389TNS
CSB-MP758389TNS
CSB-EP758389TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP758390TNS
CSB-EP758390TNS
CSB-BP758390TNS
CSB-MP758390TNS
CSB-EP758390TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Homoserine kinase (thrB) CSB-YP758391TNS
CSB-EP758391TNS
CSB-BP758391TNS
CSB-MP758391TNS
CSB-EP758391TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Uncharacterized RNA methyltransferase TT_C0988 (TT_C0988) CSB-YP758392TNS
CSB-EP758392TNS
CSB-BP758392TNS
CSB-MP758392TNS
CSB-EP758392TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus V-type ATP synthase subunit E (atpE) CSB-YP758393TNS
CSB-EP758393TNS
CSB-BP758393TNS
CSB-MP758393TNS
CSB-EP758393TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus V-type ATP synthase beta chain (atpB) CSB-YP758394TNS
CSB-EP758394TNS
CSB-BP758394TNS
CSB-MP758394TNS
CSB-EP758394TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP758395TNS
CSB-EP758395TNS
CSB-BP758395TNS
CSB-MP758395TNS
CSB-EP758395TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Maf-like protein TT_C0825 (TT_C0825) CSB-YP758396TNS
CSB-EP758396TNS
CSB-BP758396TNS
CSB-MP758396TNS
CSB-EP758396TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP758397TNS
CSB-EP758397TNS
CSB-BP758397TNS
CSB-MP758397TNS
CSB-EP758397TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP758398TNS
CSB-EP758398TNS
CSB-BP758398TNS
CSB-MP758398TNS
CSB-EP758398TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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