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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human FAST kinase domain-containing protein 5 (FASTKD5) CSB-YP759173HU
CSB-EP759173HU
CSB-BP759173HU
CSB-MP759173HU
CSB-EP759173HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Lysine-specific demethylase 3B (KDM3B), partial CSB-YP759174HU
CSB-BP759174HU
CSB-MP759174HU
CSB-EP759174HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Carbohydrate sulfotransferase 3 (CHST3), partial CSB-YP759175HU
CSB-EP759175HU
CSB-BP759175HU
CSB-MP759175HU
CSB-EP759175HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichodiene synthase (TRI5) CSB-YP759176FAAU
CSB-EP759176FAAU
CSB-BP759176FAAU
CSB-MP759176FAAU
CSB-EP759176FAAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable phospholipase C1020.13c (SPCC1020.13c, SPCC14G10.05), partial CSB-YP759177SXV
CSB-EP759177SXV
CSB-BP759177SXV
CSB-MP759177SXV
CSB-EP759177SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces paradoxus Protein URE2 (URE2) CSB-YP759178SAI
CSB-EP759178SAI
CSB-BP759178SAI
CSB-MP759178SAI
CSB-EP759178SAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Torpedo marmorata Des[Ser (1)Pro (2)] scyliorhinin-2 CSB-YP759179TGV
CSB-EP759179TGV
CSB-BP759179TGV
CSB-MP759179TGV
CSB-EP759179TGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptopelia orientalis Hemoglobin subunit beta (HBB) CSB-YP759180SHAB
CSB-EP759180SHAB
CSB-BP759180SHAB
CSB-MP759180SHAB
CSB-EP759180SHAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus atrox Kallikrein-like EII CSB-YP759181DYC
CSB-EP759181DYC
CSB-BP759181DYC
CSB-MP759181DYC
CSB-EP759181DYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tragopan satyra Lysozyme C (LYZ) CSB-YP759182THH
CSB-EP759182THH
CSB-BP759182THH
CSB-MP759182THH
CSB-EP759182THH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Catreus wallichii Lysozyme C (LYZ) CSB-YP759183CEW
CSB-EP759183CEW
CSB-BP759183CEW
CSB-MP759183CEW
CSB-EP759183CEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein kinase C beta type (prkcb), partial CSB-YP759184XBE
CSB-EP759184XBE
CSB-BP759184XBE
CSB-MP759184XBE
CSB-EP759184XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mus caroli Nucleoside diphosphate-linked moiety X motif 19, mitochondrial (Nudt19) CSB-YP759185MUU
CSB-EP759185MUU
CSB-BP759185MUU
CSB-MP759185MUU
CSB-EP759185MUU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein impA CSB-YP759186ENL
CSB-EP759186ENL
CSB-BP759186ENL
CSB-MP759186ENL
CSB-EP759186ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Chlorophyll a-b binding protein 3c, chloroplastic CSB-YP759187EWE
CSB-EP759187EWE
CSB-BP759187EWE
CSB-MP759187EWE
CSB-EP759187EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine cAMP-regulated phosphoprotein 21 (ARPP21) CSB-YP759188BO
CSB-EP759188BO
CSB-BP759188BO
CSB-MP759188BO
CSB-EP759188BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chalinolobus morio Hemoglobin subunit beta-2 (HBB2) CSB-YP759189CHM
CSB-EP759189CHM
CSB-BP759189CHM
CSB-MP759189CHM
CSB-EP759189CHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Arylacetamide deacetylase (AADAC), partial CSB-YP759190RB
CSB-EP759190RB
CSB-BP759190RB
CSB-MP759190RB
CSB-EP759190RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Corticosteroid 11-beta-dehydrogenase isozyme 1 (HSD11B1), partial CSB-YP759191RB
CSB-EP759191RB
CSB-BP759191RB
CSB-MP759191RB
CSB-EP759191RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Transposable element P transposase (T), partial CSB-YP759192DLU
CSB-EP759192DLU
CSB-BP759192DLU
CSB-MP759192DLU
CSB-EP759192DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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