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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii DASH complex subunit ASK1 (ASK1) CSB-YP758779DOT
CSB-EP758779DOT
CSB-BP758779DOT
CSB-MP758779DOT
CSB-EP758779DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Serine/threonine-protein kinase STE20 (STE20), partial CSB-YP758780DOT
CSB-EP758780DOT
CSB-BP758780DOT
CSB-MP758780DOT
CSB-EP758780DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DNA-directed RNA polymerase I subunit RPA2 (RPA2), partial CSB-YP758781DOT
CSB-EP758781DOT
CSB-BP758781DOT
CSB-MP758781DOT
CSB-EP758781DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Vacuolar membrane-associated protein IML1 (IML1), partial CSB-YP758782DOT
CSB-EP758782DOT
CSB-BP758782DOT
CSB-MP758782DOT
CSB-EP758782DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-rRNA-processing protein IPI3 (IPI3), partial CSB-YP758783DOT
CSB-EP758783DOT
CSB-BP758783DOT
CSB-MP758783DOT
CSB-EP758783DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Adenylosuccinate synthetase (ABL186W) CSB-YP758784DOT
CSB-EP758784DOT
CSB-BP758784DOT
CSB-MP758784DOT
CSB-EP758784DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein SWT21 (SWT21) CSB-YP758785DOT
CSB-EP758785DOT
CSB-BP758785DOT
CSB-MP758785DOT
CSB-EP758785DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Genetic interactor of prohibitin 7, mitochondrial (GEP7), partial CSB-YP758786DOT1
CSB-EP758786DOT1
CSB-BP758786DOT1
CSB-MP758786DOT1
CSB-EP758786DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Transcription factor IWS1 (IWS1) CSB-YP758787DOT
CSB-EP758787DOT
CSB-BP758787DOT
CSB-MP758787DOT
CSB-EP758787DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial inner membrane protease ATP23 (ATP23) CSB-YP758788DOT
CSB-EP758788DOT
CSB-BP758788DOT
CSB-MP758788DOT
CSB-EP758788DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Transcription elongation factor SPT6 (SPT6), partial CSB-YP758789DOT
CSB-EP758789DOT
CSB-BP758789DOT
CSB-MP758789DOT
CSB-EP758789DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent rRNA helicase RRP3 (RRP3) CSB-YP758790DOT
CSB-EP758790DOT
CSB-BP758790DOT
CSB-MP758790DOT
CSB-EP758790DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mediator of RNA polymerase II transcription subunit 20 (SRB2) CSB-YP758791DOT
CSB-EP758791DOT
CSB-BP758791DOT
CSB-MP758791DOT
CSB-EP758791DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Thiamine thiazole synthase (THI4) CSB-YP758792DOT
CSB-EP758792DOT
CSB-BP758792DOT
CSB-MP758792DOT
CSB-EP758792DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cytochrome c oxidase assembly protein COX16, mitochondrial (COX16), partial CSB-YP758793DOT1
CSB-EP758793DOT1
CSB-BP758793DOT1
CSB-MP758793DOT1
CSB-EP758793DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Protease HtpX homolog (htpX), partial CSB-YP758794LEV1
CSB-EP758794LEV1
CSB-BP758794LEV1
CSB-MP758794LEV1
CSB-EP758794LEV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Acyl carrier protein (acpP) CSB-YP758795LEV
CSB-EP758795LEV
CSB-BP758795LEV
CSB-MP758795LEV
CSB-EP758795LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein arginine N-methyltransferase 6.2 (PRMT6.2) CSB-YP758796OFG
CSB-EP758796OFG
CSB-BP758796OFG
CSB-MP758796OFG
CSB-EP758796OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica KH domain-containing protein SPIN1 (SPIN1) CSB-YP758797OFG
CSB-EP758797OFG
CSB-BP758797OFG
CSB-MP758797OFG
CSB-EP758797OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica DEAD-box ATP-dependent RNA helicase 37 (PL10A), partial CSB-YP758798OFG
CSB-EP758798OFG
CSB-BP758798OFG
CSB-MP758798OFG
CSB-EP758798OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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