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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii 37S ribosomal protein S25, mitochondrial (RSM25) CSB-YP758759DOT
CSB-EP758759DOT
CSB-BP758759DOT
CSB-MP758759DOT
CSB-EP758759DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii tRNA (adenine (58)-N (1))-methyltransferase non-catalytic subunit TRM6 (TRM6) CSB-YP758760DOT
CSB-EP758760DOT
CSB-BP758760DOT
CSB-MP758760DOT
CSB-EP758760DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Genetic interactor of prohibitins 3, mitochondrial (GEP3), partial CSB-YP758761DOT
CSB-EP758761DOT
CSB-BP758761DOT
CSB-MP758761DOT
CSB-EP758761DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Hsp70 nucleotide exchange factor FES1 (FES1) CSB-YP758762DOT
CSB-EP758762DOT
CSB-BP758762DOT
CSB-MP758762DOT
CSB-EP758762DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Endoplasmic oxidoreductin-1 (ERO1), partial CSB-YP758763DOT
CSB-EP758763DOT
CSB-BP758763DOT
CSB-MP758763DOT
CSB-EP758763DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Guanine nucleotide exchange factor LTE1 (LTE1), partial CSB-YP758764DOT
CSB-EP758764DOT
CSB-BP758764DOT
CSB-MP758764DOT
CSB-EP758764DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 40S ribosomal protein S21 (RPS21) CSB-YP758765DOT
CSB-EP758765DOT
CSB-BP758765DOT
CSB-MP758765DOT
CSB-EP758765DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent RNA helicase DHH1 (DHH1) CSB-YP758766DOT
CSB-EP758766DOT
CSB-BP758766DOT
CSB-MP758766DOT
CSB-EP758766DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DNA-directed RNA polymerases I, II, and III subunit RPABC5 (RPB10) CSB-YP758767DOT
CSB-EP758767DOT
CSB-BP758767DOT
CSB-MP758767DOT
CSB-EP758767DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial holo-[acyl-carrier-protein] synthase (PPT2) CSB-YP758768DOT
CSB-EP758768DOT
CSB-BP758768DOT
CSB-MP758768DOT
CSB-EP758768DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Plasma membrane proteolipid 3 (PMP3), partial CSB-YP758769DOT
CSB-EP758769DOT
CSB-BP758769DOT
CSB-MP758769DOT
CSB-EP758769DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 78 kDa glucose-regulated protein homolog (KAR2), partial CSB-YP758770DOT
CSB-EP758770DOT
CSB-BP758770DOT
CSB-MP758770DOT
CSB-EP758770DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Coenzyme Q-binding protein COQ10, mitochondrial (COQ10) CSB-YP758771DOT
CSB-EP758771DOT
CSB-BP758771DOT
CSB-MP758771DOT
CSB-EP758771DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Dihydroorotate dehydrogenase (quinone), mitochondrial (URA9), partial CSB-YP758772DOT
CSB-EP758772DOT
CSB-BP758772DOT
CSB-MP758772DOT
CSB-EP758772DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Stress response protein NST1 (NST1), partial CSB-YP758773DOT
CSB-EP758773DOT
CSB-BP758773DOT
CSB-MP758773DOT
CSB-EP758773DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 40S ribosomal protein S0 (RPS0) CSB-YP758774DOT
CSB-EP758774DOT
CSB-BP758774DOT
CSB-MP758774DOT
CSB-EP758774DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii rRNA-processing protein FYV7 (FYV7) CSB-YP758775DOT
CSB-EP758775DOT
CSB-BP758775DOT
CSB-MP758775DOT
CSB-EP758775DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Translocation protein SEC62 (SEC62), partial CSB-YP758776DOT1
CSB-EP758776DOT1
CSB-BP758776DOT1
CSB-MP758776DOT1
CSB-EP758776DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Carbamoyl-phosphate synthase arginine-specific large chain (CPA2), partial CSB-YP758777DOT
CSB-EP758777DOT
CSB-BP758777DOT
CSB-MP758777DOT
CSB-EP758777DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Nucleolar GTP-binding protein 2 (NOG2), partial CSB-YP758778DOT
CSB-EP758778DOT
CSB-BP758778DOT
CSB-MP758778DOT
CSB-EP758778DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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