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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria innocua serovar 6a 50S ribosomal protein L14 (rplN) CSB-YP758960LGU
CSB-EP758960LGU
CSB-BP758960LGU
CSB-MP758960LGU
CSB-EP758960LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a UPF0356 protein lin1027 (lin1027) CSB-YP758961LGU
CSB-EP758961LGU
CSB-BP758961LGU
CSB-MP758961LGU
CSB-EP758961LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N-acyl homoserine lactonase (aiiA) CSB-YP758962BDAX
CSB-EP758962BDAX
CSB-BP758962BDAX
CSB-MP758962BDAX
CSB-EP758962BDAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP758963YAH
CSB-EP758963YAH
CSB-BP758963YAH
CSB-MP758963YAH
CSB-EP758963YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-oxoglutarate-dependent ethylene/succinate-forming enzyme (efe) CSB-YP758964PHAX
CSB-EP758964PHAX
CSB-BP758964PHAX
CSB-MP758964PHAX
CSB-EP758964PHAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication regulatory protein repA2 (repA2) CSB-YP758965EOD
CSB-EP758965EOD
CSB-BP758965EOD
CSB-MP758965EOD
CSB-EP758965EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA replication and repair protein recF (recF) CSB-YP758966SZB
CSB-EP758966SZB
CSB-BP758966SZB
CSB-MP758966SZB
CSB-EP758966SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitamin B12 import system permease protein BtuC (btuC), partial CSB-YP758967SZB
CSB-EP758967SZB
CSB-BP758967SZB
CSB-MP758967SZB
CSB-EP758967SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylose isomerase (xylA) CSB-YP758968SWW
CSB-EP758968SWW
CSB-BP758968SWW
CSB-MP758968SWW
CSB-EP758968SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Chaperone protein ClpB (clpB), partial CSB-YP758969BMQ
CSB-EP758969BMQ
CSB-BP758969BMQ
CSB-MP758969BMQ
CSB-EP758969BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L6 (rplF) CSB-YP758970YAS
CSB-EP758970YAS
CSB-BP758970YAS
CSB-MP758970YAS
CSB-EP758970YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autoinducer 2 import system permease protein lsrD (lsrD), partial CSB-YP758971YAS
CSB-EP758971YAS
CSB-BP758971YAS
CSB-MP758971YAS
CSB-EP758971YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0441 protein YPO0661/y3517/YP_2976 (YPO0661, y3517, YP_2976) CSB-YP758972YAS
CSB-EP758972YAS
CSB-BP758972YAS
CSB-MP758972YAS
CSB-EP758972YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein grpE (grpE) CSB-YP758973YAS
CSB-EP758973YAS
CSB-BP758973YAS
CSB-MP758973YAS
CSB-EP758973YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein ZapC (zapC) CSB-YP758974YAS
CSB-EP758974YAS
CSB-BP758974YAS
CSB-MP758974YAS
CSB-EP758974YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deferrochelatase/peroxidase EfeB (efeB) CSB-YP758975YAS
CSB-EP758975YAS
CSB-BP758975YAS
CSB-MP758975YAS
CSB-EP758975YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0745 protein YPO2080/y2231/YP_1923 (YPO2080, y2231, YP_1923) CSB-YP758976YAS
CSB-EP758976YAS
CSB-BP758976YAS
CSB-MP758976YAS
CSB-EP758976YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillin-binding protein activator LpoB (lpoB) CSB-YP758977YAS
CSB-EP758977YAS
CSB-BP758977YAS
CSB-MP758977YAS
CSB-EP758977YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP758978YAS
CSB-EP758978YAS
CSB-BP758978YAS
CSB-MP758978YAS
CSB-EP758978YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative multidrug resistance protein MdtD (mdtD), partial CSB-YP758979YAS
CSB-EP758979YAS
CSB-BP758979YAS
CSB-MP758979YAS
CSB-EP758979YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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