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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant GTPase obg (obg) CSB-YP758980YAS
CSB-EP758980YAS
CSB-BP758980YAS
CSB-MP758980YAS
CSB-EP758980YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA ligase B (ligB), partial CSB-YP758981YAS
CSB-EP758981YAS
CSB-BP758981YAS
CSB-MP758981YAS
CSB-EP758981YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L13 (rplM) CSB-YP758982XAY
CSB-EP758982XAY
CSB-BP758982XAY
CSB-MP758982XAY
CSB-EP758982XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0340 protein spyM18_1963 (spyM18_1963) CSB-YP758983SMU
CSB-EP758983SMU
CSB-BP758983SMU
CSB-MP758983SMU
CSB-EP758983SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthine phosphoribosyltransferase (xpt) CSB-YP758984SMU
CSB-EP758984SMU
CSB-BP758984SMU
CSB-MP758984SMU
CSB-EP758984SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L15 (rplO) CSB-YP758985SMU
CSB-EP758985SMU
CSB-BP758985SMU
CSB-MP758985SMU
CSB-EP758985SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L22 (rplV) CSB-YP758986SMU
CSB-EP758986SMU
CSB-BP758986SMU
CSB-MP758986SMU
CSB-EP758986SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0386 protein yjhX (yjhX) CSB-YP758987SXB
CSB-EP758987SXB
CSB-BP758987SXB
CSB-MP758987SXB
CSB-EP758987SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0337 protein yjbJ (yjbJ) CSB-YP758988SXB
CSB-EP758988SXB
CSB-BP758988SXB
CSB-MP758988SXB
CSB-EP758988SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium ATP synthase subunit beta (atpD) CSB-YP758989SXB
CSB-EP758989SXB
CSB-BP758989SXB
CSB-MP758989SXB
CSB-EP758989SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Protein-export protein SecB (secB) CSB-YP758990SXB
CSB-EP758990SXB
CSB-BP758990SXB
CSB-MP758990SXB
CSB-EP758990SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 50S ribosomal protein L17 (rplQ) CSB-YP758991SXB
CSB-EP758991SXB
CSB-BP758991SXB
CSB-MP758991SXB
CSB-EP758991SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP758992SXB
CSB-EP758992SXB
CSB-BP758992SXB
CSB-MP758992SXB
CSB-EP758992SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium tRNA-specific adenosine deaminase (tadA) CSB-YP758993SXB
CSB-EP758993SXB
CSB-BP758993SXB
CSB-MP758993SXB
CSB-EP758993SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP758994SXB1
CSB-EP758994SXB1
CSB-BP758994SXB1
CSB-MP758994SXB1
CSB-EP758994SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA), partial CSB-YP758995SXB1
CSB-EP758995SXB1
CSB-BP758995SXB1
CSB-MP758995SXB1
CSB-EP758995SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0482 protein ynfB (ynfB) CSB-YP758996SXB
CSB-EP758996SXB
CSB-BP758996SXB
CSB-MP758996SXB
CSB-EP758996SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Probable phosphatase YcdX (ycdX) CSB-YP758997SXB
CSB-EP758997SXB
CSB-BP758997SXB
CSB-MP758997SXB
CSB-EP758997SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Dipeptide permease D (dtpD), partial CSB-YP758998SXB
CSB-EP758998SXB
CSB-BP758998SXB
CSB-MP758998SXB
CSB-EP758998SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Acyl carrier protein phosphodiesterase (acpH) CSB-YP758999SXB
CSB-EP758999SXB
CSB-BP758999SXB
CSB-MP758999SXB
CSB-EP758999SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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