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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Ajellomyces capsulata NADH-cytochrome b5 reductase 2 (MCR1), partial CSB-YP006319AUN1
CSB-EP006319AUN1
CSB-BP006319AUN1
CSB-MP006319AUN1
CSB-EP006319AUN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces capsulata Structure-specific endonuclease subunit SLX4 (SLX4), partial CSB-YP002843AUN
CSB-EP002843AUN
CSB-BP002843AUN
CSB-MP002843AUN
CSB-EP002843AUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana H/ACA ribonucleoprotein complex subunit 1 (GAR1) CSB-YP009257BVD
CSB-EP009257BVD
CSB-BP009257BVD
CSB-MP009257BVD
CSB-EP009257BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Enolase-phosphatase E1 (utr4) CSB-YP007674BVD
CSB-EP007674BVD
CSB-BP007674BVD
CSB-MP007674BVD
CSB-EP007674BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. ATP synthase subunit a (atpB), partial CSB-YP015070JAQ1
CSB-EP015070JAQ1
CSB-BP015070JAQ1
CSB-MP015070JAQ1
CSB-EP015070JAQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Elongation factor Ts (tsf) CSB-YP025124KAX
CSB-EP025124KAX
CSB-BP025124KAX
CSB-MP025124KAX
CSB-EP025124KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Enolase-phosphatase E1 (mtnC) CSB-YP007674KAX
CSB-EP007674KAX
CSB-BP007674KAX
CSB-MP007674KAX
CSB-EP007674KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Peptide chain release factor 1 (prfA) CSB-YP015197KAX
CSB-EP015197KAX
CSB-BP015197KAX
CSB-MP015197KAX
CSB-EP015197KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Malate dehydrogenase (mdh) CSB-YP013623KAX
CSB-EP013623KAX
CSB-BP013623KAX
CSB-MP013623KAX
CSB-EP013623KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Methionyl-tRNA formyltransferase (fmt) CSB-YP015150KAX
CSB-EP015150KAX
CSB-BP015150KAX
CSB-MP015150KAX
CSB-EP015150KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 6-phosphofructokinase (pfkA) CSB-YP017822KAX
CSB-EP017822KAX
CSB-BP017822KAX
CSB-MP017822KAX
CSB-EP017822KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae tRNA dimethylallyltransferase (miaA) CSB-YP024529KAX
CSB-EP024529KAX
CSB-BP024529KAX
CSB-MP024529KAX
CSB-EP024529KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 6-phosphofructokinase (pfkA) CSB-YP017822AUP
CSB-EP017822AUP
CSB-BP017822AUP
CSB-MP017822AUP
CSB-EP017822AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Lipoyl synthase (lipA) CSB-YP012927AUP
CSB-EP012927AUP
CSB-BP012927AUP
CSB-MP012927AUP
CSB-EP012927AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Elongation factor G (fusA), partial CSB-YP009373AUP
CSB-EP009373AUP
CSB-BP009373AUP
CSB-MP009373AUP
CSB-EP009373AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917SUI
CSB-EP022917SUI
CSB-BP022917SUI
CSB-MP022917SUI
CSB-EP022917SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit b (atpF), partial CSB-YP002358SUI1
CSB-EP002358SUI1
CSB-BP002358SUI1
CSB-MP002358SUI1
CSB-EP002358SUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae tRNA pseudouridine synthase A (truA) CSB-YP019086STV
CSB-EP019086STV
CSB-BP019086STV
CSB-MP019086STV
CSB-EP019086STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451STV
CSB-EP015451STV
CSB-BP015451STV
CSB-MP015451STV
CSB-EP015451STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Lipoyl synthase (lipA) CSB-YP012927STV
CSB-EP012927STV
CSB-BP012927STV
CSB-MP012927STV
CSB-EP012927STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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