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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brugia malayi Flap endonuclease 1 (FEN1) CSB-YP008585BWV
CSB-EP008585BWV
CSB-BP008585BWV
CSB-MP008585BWV
CSB-EP008585BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi F-box/SPRY domain-containing protein 1 (Bm1_56115) CSB-YP008510BWV
CSB-EP008510BWV
CSB-BP008510BWV
CSB-MP008510BWV
CSB-EP008510BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Myeloid differentiation primary response protein MyD88 (myd88) CSB-YP015282TKY
CSB-EP015282TKY
CSB-BP015282TKY
CSB-MP015282TKY
CSB-EP015282TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Coiled-coil domain-containing protein 89 (ccdc89) CSB-YP004758DIL
CSB-EP004758DIL
CSB-BP004758DIL
CSB-MP004758DIL
CSB-EP004758DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Collagen and calcium-binding EGF domain-containing protein 1 (ccbe1) CSB-YP004615DIL
CSB-EP004615DIL
CSB-BP004615DIL
CSB-MP004615DIL
CSB-EP004615DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Sodium/glucose cotransporter 4 (slc5a9), partial CSB-YP021686DIL
CSB-EP021686DIL
CSB-BP021686DIL
CSB-MP021686DIL
CSB-EP021686DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dynactin subunit 6 (CBG04171) CSB-YP006569CXX
CSB-EP006569CXX
CSB-BP006569CXX
CSB-MP006569CXX
CSB-EP006569CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nuclear cap-binding protein subunit 1 (ncbp-1), partial CSB-YP015520CXX
CSB-EP015520CXX
CSB-BP015520CXX
CSB-MP015520CXX
CSB-EP015520CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Choline transporter-like protein 1 (chtl-1), partial CSB-YP021648CXX
CSB-EP021648CXX
CSB-BP021648CXX
CSB-MP021648CXX
CSB-EP021648CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant TATA box-binding protein-associated factor RNA polymerase I subunit B (CBG15174), partial CSB-YP023084CXX
CSB-EP023084CXX
CSB-BP023084CXX
CSB-MP023084CXX
CSB-EP023084CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 40S ribosomal protein S3a (rps-1) CSB-YP020444CXX
CSB-EP020444CXX
CSB-BP020444CXX
CSB-MP020444CXX
CSB-EP020444CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin--tyrosine ligase-like protein 12 (ttll-12), partial CSB-YP025256CXX
CSB-EP025256CXX
CSB-BP025256CXX
CSB-MP025256CXX
CSB-EP025256CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein N-terminal glutamine amidohydrolase (CBG21918) CSB-YP026083CXX
CSB-EP026083CXX
CSB-BP026083CXX
CSB-MP026083CXX
CSB-EP026083CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kynurenine 3-monooxygenase (CBG23368), partial CSB-YP012475CXX1
CSB-EP012475CXX1
CSB-BP012475CXX1
CSB-MP012475CXX1
CSB-EP012475CXX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Thymidylate kinase (tmk) CSB-YP007226LLE
CSB-EP007226LLE
CSB-BP007226LLE
CSB-MP007226LLE
CSB-EP007226LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Triosephosphate isomerase (tpiA) CSB-YP024102LLE
CSB-EP024102LLE
CSB-BP024102LLE
CSB-MP024102LLE
CSB-EP024102LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Peptide chain release factor 1 (prfA) CSB-YP015197LLE
CSB-EP015197LLE
CSB-BP015197LLE
CSB-MP015197LLE
CSB-EP015197LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus ATP synthase subunit b (atpF), partial CSB-YP002358LLE1
CSB-EP002358LLE1
CSB-BP002358LLE1
CSB-MP002358LLE1
CSB-EP002358LLE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Peptide deformylase (def) CSB-YP017707LLE
CSB-EP017707LLE
CSB-BP017707LLE
CSB-MP017707LLE
CSB-EP017707LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Ribosome-recycling factor (frr) CSB-YP014941LLE
CSB-EP014941LLE
CSB-BP014941LLE
CSB-MP014941LLE
CSB-EP014941LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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