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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP802314BAM
CSB-EP802314BAM
CSB-BP802314BAM
CSB-MP802314BAM
CSB-EP802314BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP802315BAM
CSB-EP802315BAM
CSB-BP802315BAM
CSB-MP802315BAM
CSB-EP802315BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication protein E1 (E1), partial CSB-YP802316HEAM
CSB-EP802316HEAM
CSB-BP802316HEAM
CSB-MP802316HEAM
CSB-EP802316HEAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP802317BAM
CSB-EP802317BAM
CSB-BP802317BAM
CSB-MP802317BAM
CSB-EP802317BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP802318BAM
CSB-EP802318BAM
CSB-BP802318BAM
CSB-MP802318BAM
CSB-EP802318BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0358 protein BC_3949 (BC_3949) CSB-YP802319BAM
CSB-EP802319BAM
CSB-BP802319BAM
CSB-MP802319BAM
CSB-EP802319BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Uncharacterized N-acetyltransferase BC_3921 (BC_3921) CSB-YP802320BAM
CSB-EP802320BAM
CSB-BP802320BAM
CSB-MP802320BAM
CSB-EP802320BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cell division protein DivIB (divIB), partial CSB-YP802321BAM
CSB-EP802321BAM
CSB-BP802321BAM
CSB-MP802321BAM
CSB-EP802321BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP802322BAM
CSB-EP802322BAM
CSB-BP802322BAM
CSB-MP802322BAM
CSB-EP802322BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Guanylate kinase (gmk) CSB-YP802323BAM
CSB-EP802323BAM
CSB-BP802323BAM
CSB-MP802323BAM
CSB-EP802323BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP802324BAM
CSB-EP802324BAM
CSB-BP802324BAM
CSB-MP802324BAM
CSB-EP802324BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S16 (rpsP) CSB-YP802325BAM
CSB-EP802325BAM
CSB-BP802325BAM
CSB-MP802325BAM
CSB-EP802325BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent protease subunit HslV (hslV) CSB-YP802326BAM
CSB-EP802326BAM
CSB-BP802326BAM
CSB-MP802326BAM
CSB-EP802326BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S15 (rpsO) CSB-YP802327BAM
CSB-EP802327BAM
CSB-BP802327BAM
CSB-MP802327BAM
CSB-EP802327BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Peptidase T (pepT) CSB-YP802328BAM
CSB-EP802328BAM
CSB-BP802328BAM
CSB-MP802328BAM
CSB-EP802328BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Imidazolonepropionase (hutI) CSB-YP802329BAM
CSB-EP802329BAM
CSB-BP802329BAM
CSB-MP802329BAM
CSB-EP802329BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein tlp (tlp) CSB-YP802330BAM
CSB-EP802330BAM
CSB-BP802330BAM
CSB-MP802330BAM
CSB-EP802330BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0316 protein BC_3353 (BC_3353), partial CSB-YP802331BAM1
CSB-EP802331BAM1
CSB-BP802331BAM1
CSB-MP802331BAM1
CSB-EP802331BAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutaminase 2 (glsA2) CSB-YP802332BAM
CSB-EP802332BAM
CSB-BP802332BAM
CSB-MP802332BAM
CSB-EP802332BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable malate:quinone oxidoreductase (mqo) CSB-YP802333BAM
CSB-EP802333BAM
CSB-BP802333BAM
CSB-MP802333BAM
CSB-EP802333BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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