Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas syringae pv. tomato Anthranilate phosphoribosyltransferase (trpD) CSB-YP803435FGP
CSB-EP803435FGP
CSB-BP803435FGP
CSB-MP803435FGP
CSB-EP803435FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Protein ApaG (apaG) CSB-YP803436FGP
CSB-EP803436FGP
CSB-BP803436FGP
CSB-MP803436FGP
CSB-EP803436FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP803437FGP
CSB-EP803437FGP
CSB-BP803437FGP
CSB-MP803437FGP
CSB-EP803437FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 8-amino-7-oxononanoate synthase (bioF) CSB-YP803438FGP
CSB-EP803438FGP
CSB-BP803438FGP
CSB-MP803438FGP
CSB-EP803438FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP803439FGP1
CSB-EP803439FGP1
CSB-BP803439FGP1
CSB-MP803439FGP1
CSB-EP803439FGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato DNA ligase B (ligB), partial CSB-YP803440FGP
CSB-EP803440FGP
CSB-BP803440FGP
CSB-MP803440FGP
CSB-EP803440FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Glycine cleavage system H protein 2 (gcvH2) CSB-YP803441FGP
CSB-EP803441FGP
CSB-BP803441FGP
CSB-MP803441FGP
CSB-EP803441FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 33 kDa chaperonin (hslO) CSB-YP803442FGP
CSB-EP803442FGP
CSB-BP803442FGP
CSB-MP803442FGP
CSB-EP803442FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP803443FGP
CSB-EP803443FGP
CSB-BP803443FGP
CSB-MP803443FGP
CSB-EP803443FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Tryptophan synthase beta chain (trpB) CSB-YP803444FGP
CSB-EP803444FGP
CSB-BP803444FGP
CSB-MP803444FGP
CSB-EP803444FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato D-amino acid dehydrogenase small subunit (dadA) CSB-YP803445FGP
CSB-EP803445FGP
CSB-BP803445FGP
CSB-MP803445FGP
CSB-EP803445FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Orotate phosphoribosyltransferase (pyrE) CSB-YP803446FGP
CSB-EP803446FGP
CSB-BP803446FGP
CSB-MP803446FGP
CSB-EP803446FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida ATP synthase subunit b (atpF), partial CSB-YP803447FGB1
CSB-EP803447FGB1
CSB-BP803447FGB1
CSB-MP803447FGB1
CSB-EP803447FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Bifunctional protein GlmU (glmU) CSB-YP803448FGB
CSB-EP803448FGB
CSB-BP803448FGB
CSB-MP803448FGB
CSB-EP803448FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP803449FGB
CSB-EP803449FGB
CSB-BP803449FGB
CSB-MP803449FGB
CSB-EP803449FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Orotate phosphoribosyltransferase (pyrE) CSB-YP803450FGB
CSB-EP803450FGB
CSB-BP803450FGB
CSB-MP803450FGB
CSB-EP803450FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Xanthine phosphoribosyltransferase (xpt) CSB-YP803451FGB
CSB-EP803451FGB
CSB-BP803451FGB
CSB-MP803451FGB
CSB-EP803451FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0178 protein PP_5221 (PP_5221) CSB-YP803452FGB
CSB-EP803452FGB
CSB-BP803452FGB
CSB-MP803452FGB
CSB-EP803452FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida RNA pyrophosphohydrolase (rppH) CSB-YP803453FGB
CSB-EP803453FGB
CSB-BP803453FGB
CSB-MP803453FGB
CSB-EP803453FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Thiazole synthase (thiG) CSB-YP803454FGB
CSB-EP803454FGB
CSB-BP803454FGB
CSB-MP803454FGB
CSB-EP803454FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>