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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermosynechococcus elongatus Chaperone protein dnaK1 (dnaK1), partial CSB-YP807823TDQ
CSB-EP807823TDQ
CSB-BP807823TDQ
CSB-MP807823TDQ
CSB-EP807823TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Heat-inducible transcription repressor HrcA (hrcA) CSB-YP807824TDQ
CSB-EP807824TDQ
CSB-BP807824TDQ
CSB-MP807824TDQ
CSB-EP807824TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Glucose-6-phosphate isomerase (pgi), partial CSB-YP807825TDQ
CSB-EP807825TDQ
CSB-BP807825TDQ
CSB-MP807825TDQ
CSB-EP807825TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Ribonuclease HII (rnhB) CSB-YP807826TDQ
CSB-EP807826TDQ
CSB-BP807826TDQ
CSB-MP807826TDQ
CSB-EP807826TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP807827TDQ
CSB-EP807827TDQ
CSB-BP807827TDQ
CSB-MP807827TDQ
CSB-EP807827TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP807828TDQ
CSB-EP807828TDQ
CSB-BP807828TDQ
CSB-MP807828TDQ
CSB-EP807828TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus D-alanine--D-alanine ligase (ddl) CSB-YP807829TDQ
CSB-EP807829TDQ
CSB-BP807829TDQ
CSB-MP807829TDQ
CSB-EP807829TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Photosystem II reaction center Psb28 protein (psb28) CSB-YP807830TDQ
CSB-EP807830TDQ
CSB-BP807830TDQ
CSB-MP807830TDQ
CSB-EP807830TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus ATP synthase subunit delta (atpH) CSB-YP807831TDQ
CSB-EP807831TDQ
CSB-BP807831TDQ
CSB-MP807831TDQ
CSB-EP807831TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Probable 2-phosphosulfolactate phosphatase (comB) CSB-YP807832TDQ
CSB-EP807832TDQ
CSB-BP807832TDQ
CSB-MP807832TDQ
CSB-EP807832TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Phosphomethylpyrimidine synthase (thiC) CSB-YP807833TDQ
CSB-EP807833TDQ
CSB-BP807833TDQ
CSB-MP807833TDQ
CSB-EP807833TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 50S ribosomal protein L7/L12 (rplL) CSB-YP807834TDQ
CSB-EP807834TDQ
CSB-BP807834TDQ
CSB-MP807834TDQ
CSB-EP807834TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Ribosome-recycling factor (frr) CSB-YP807835TDQ
CSB-EP807835TDQ
CSB-BP807835TDQ
CSB-MP807835TDQ
CSB-EP807835TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP807836TDQ
CSB-EP807836TDQ
CSB-BP807836TDQ
CSB-MP807836TDQ
CSB-EP807836TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP807837TDQ
CSB-EP807837TDQ
CSB-BP807837TDQ
CSB-MP807837TDQ
CSB-EP807837TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 30S ribosomal protein S13 (rpsM) CSB-YP807838TDQ
CSB-EP807838TDQ
CSB-BP807838TDQ
CSB-MP807838TDQ
CSB-EP807838TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 50S ribosomal protein L29 (rpmC) CSB-YP807839TDQ
CSB-EP807839TDQ
CSB-BP807839TDQ
CSB-MP807839TDQ
CSB-EP807839TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP807840TDQ
CSB-EP807840TDQ
CSB-BP807840TDQ
CSB-MP807840TDQ
CSB-EP807840TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 30S ribosomal protein S21 (rpsU) CSB-YP807841TDQ
CSB-EP807841TDQ
CSB-BP807841TDQ
CSB-MP807841TDQ
CSB-EP807841TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae L-fucose isomerase (fucI), partial CSB-YP807842SMP
CSB-EP807842SMP
CSB-BP807842SMP
CSB-MP807842SMP
CSB-EP807842SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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