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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus epidermidis Phosphopantetheine adenylyltransferase (coaD) CSB-YP807623SLD
CSB-EP807623SLD
CSB-BP807623SLD
CSB-MP807623SLD
CSB-EP807623SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0356 protein SE_0788 (SE_0788) CSB-YP807624SLD
CSB-EP807624SLD
CSB-BP807624SLD
CSB-MP807624SLD
CSB-EP807624SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Bifunctional purine biosynthesis protein PurH (purH) CSB-YP807625SLD
CSB-EP807625SLD
CSB-BP807625SLD
CSB-MP807625SLD
CSB-EP807625SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP807626SLD
CSB-EP807626SLD
CSB-BP807626SLD
CSB-MP807626SLD
CSB-EP807626SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Glucose-6-phosphate isomerase (pgi) CSB-YP807627SLD
CSB-EP807627SLD
CSB-BP807627SLD
CSB-MP807627SLD
CSB-EP807627SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0337 protein SE_0604 (SE_0604) CSB-YP807628SLD
CSB-EP807628SLD
CSB-BP807628SLD
CSB-MP807628SLD
CSB-EP807628SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative 5' (3')-deoxyribonucleotidase (SE_0505) CSB-YP807629SLD
CSB-EP807629SLD
CSB-BP807629SLD
CSB-MP807629SLD
CSB-EP807629SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0272 protein SE_0371 (SE_0371) CSB-YP807630SLD
CSB-EP807630SLD
CSB-BP807630SLD
CSB-MP807630SLD
CSB-EP807630SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Protein vraC (SE_0347) CSB-YP807631SLD
CSB-EP807631SLD
CSB-BP807631SLD
CSB-MP807631SLD
CSB-EP807631SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 50S ribosomal protein L7/L12 (rplL) CSB-YP807632SLD
CSB-EP807632SLD
CSB-BP807632SLD
CSB-MP807632SLD
CSB-EP807632SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative TrmH family tRNA/rRNA methyltransferase (SE_0294) CSB-YP807633SLD
CSB-EP807633SLD
CSB-BP807633SLD
CSB-MP807633SLD
CSB-EP807633SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Formate acetyltransferase (pflB), partial CSB-YP807634SLD
CSB-EP807634SLD
CSB-BP807634SLD
CSB-MP807634SLD
CSB-EP807634SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Alkylmercury lyase (merB) CSB-YP807635SLD
CSB-EP807635SLD
CSB-BP807635SLD
CSB-MP807635SLD
CSB-EP807635SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis 3'-5' exoribonuclease yhaM (yhaM) CSB-YP807636OAB
CSB-EP807636OAB
CSB-BP807636OAB
CSB-MP807636OAB
CSB-EP807636OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP807637OAB
CSB-EP807637OAB
CSB-BP807637OAB
CSB-MP807637OAB
CSB-EP807637OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Phosphonates import ATP-binding protein PhnC 2 (phnC2) CSB-YP807638OAB
CSB-EP807638OAB
CSB-BP807638OAB
CSB-MP807638OAB
CSB-EP807638OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Regulatory protein recX (recX) CSB-YP807639OAB
CSB-EP807639OAB
CSB-BP807639OAB
CSB-MP807639OAB
CSB-EP807639OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Soluble cytochrome b562 (cybC) CSB-YP807640EGX
CSB-EP807640EGX
CSB-BP807640EGX
CSB-MP807640EGX
CSB-EP807640EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multidrug resistance protein MdtE (mdtE) CSB-YP807641EGX
CSB-EP807641EGX
CSB-BP807641EGX
CSB-MP807641EGX
CSB-EP807641EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trimethylamine-N-oxide reductase 2 (torZ) CSB-YP807642EGX
CSB-EP807642EGX
CSB-BP807642EGX
CSB-MP807642EGX
CSB-EP807642EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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