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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermosynechococcus elongatus Elongation factor G (fusA), partial CSB-YP807803TDQ
CSB-EP807803TDQ
CSB-BP807803TDQ
CSB-MP807803TDQ
CSB-EP807803TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 2 (acsF2) CSB-YP807804TDQ
CSB-EP807804TDQ
CSB-BP807804TDQ
CSB-MP807804TDQ
CSB-EP807804TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Sulfate/thiosulfate import ATP-binding protein CysA (cysA) CSB-YP807805TDQ
CSB-EP807805TDQ
CSB-BP807805TDQ
CSB-MP807805TDQ
CSB-EP807805TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Peptide deformylase (def) CSB-YP807806TDQ
CSB-EP807806TDQ
CSB-BP807806TDQ
CSB-MP807806TDQ
CSB-EP807806TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Translation initiation factor IF-3 (infC) CSB-YP807807TDQ
CSB-EP807807TDQ
CSB-BP807807TDQ
CSB-MP807807TDQ
CSB-EP807807TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Probable rRNA maturation factor (tlr1585) CSB-YP807808TDQ
CSB-EP807808TDQ
CSB-BP807808TDQ
CSB-MP807808TDQ
CSB-EP807808TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Photosystem II reaction center protein L (psbL), partial CSB-YP807809TDQ
CSB-EP807809TDQ
CSB-BP807809TDQ
CSB-MP807809TDQ
CSB-EP807809TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Valine--tRNA ligase (valS), partial CSB-YP807810TDQ
CSB-EP807810TDQ
CSB-BP807810TDQ
CSB-MP807810TDQ
CSB-EP807810TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Chaperone protein ClpB 1 (clpB1), partial CSB-YP807811TDQ
CSB-EP807811TDQ
CSB-BP807811TDQ
CSB-MP807811TDQ
CSB-EP807811TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Adenylyl-sulfate kinase (cysC) CSB-YP807812TDQ
CSB-EP807812TDQ
CSB-BP807812TDQ
CSB-MP807812TDQ
CSB-EP807812TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Protein grpE (grpE) CSB-YP807813TDQ
CSB-EP807813TDQ
CSB-BP807813TDQ
CSB-MP807813TDQ
CSB-EP807813TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus NAD (P)H-quinone oxidoreductase subunit H (ndhH) CSB-YP807814TDQ
CSB-EP807814TDQ
CSB-BP807814TDQ
CSB-MP807814TDQ
CSB-EP807814TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Cell division protein SepF (sepF) CSB-YP807815TDQ
CSB-EP807815TDQ
CSB-BP807815TDQ
CSB-MP807815TDQ
CSB-EP807815TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 30S ribosomal protein S20 (rpsT) CSB-YP807816TDQ
CSB-EP807816TDQ
CSB-BP807816TDQ
CSB-MP807816TDQ
CSB-EP807816TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Aspartate--tRNA ligase (aspS), partial CSB-YP807817TDQ
CSB-EP807817TDQ
CSB-BP807817TDQ
CSB-MP807817TDQ
CSB-EP807817TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Putative diflavin flavoprotein A 1 (dfa1), partial CSB-YP807818TDQ
CSB-EP807818TDQ
CSB-BP807818TDQ
CSB-MP807818TDQ
CSB-EP807818TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP807819TDQ
CSB-EP807819TDQ
CSB-BP807819TDQ
CSB-MP807819TDQ
CSB-EP807819TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP807820TDQ
CSB-EP807820TDQ
CSB-BP807820TDQ
CSB-MP807820TDQ
CSB-EP807820TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP807821TDQ
CSB-EP807821TDQ
CSB-BP807821TDQ
CSB-MP807821TDQ
CSB-EP807821TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Arginine--tRNA ligase (argS), partial CSB-YP807822TDQ
CSB-EP807822TDQ
CSB-BP807822TDQ
CSB-MP807822TDQ
CSB-EP807822TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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