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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acetobacter pasteurianus 10 kDa chaperonin (groS) CSB-YP808383AVX
CSB-EP808383AVX
CSB-BP808383AVX
CSB-MP808383AVX
CSB-EP808383AVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamyl-tRNA reductase (hemA) CSB-YP808384BQV
CSB-EP808384BQV
CSB-BP808384BQV
CSB-MP808384BQV
CSB-EP808384BQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hut operon positive regulatory protein (hutP) CSB-YP808385BQJ
CSB-EP808385BQJ
CSB-BP808385BQJ
CSB-MP808385BQJ
CSB-EP808385BQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Redox-sensing transcriptional repressor rex (rex) CSB-YP808386LNF
CSB-EP808386LNF
CSB-BP808386LNF
CSB-MP808386LNF
CSB-EP808386LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP808387HCT
CSB-EP808387HCT
CSB-BP808387HCT
CSB-MP808387HCT
CSB-EP808387HCT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens UPF0264 protein ORF22 CSB-YP808388MFK
CSB-EP808388MFK
CSB-BP808388MFK
CSB-MP808388MFK
CSB-EP808388MFK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens Dipeptide and tripeptide permease B (dtpB), partial CSB-YP808389PII
CSB-EP808389PII
CSB-BP808389PII
CSB-MP808389PII
CSB-EP808389PII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila abortus Chaperone protein DnaK (dnaK), partial CSB-YP808390CJA
CSB-EP808390CJA
CSB-BP808390CJA
CSB-MP808390CJA
CSB-EP808390CJA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoglycerate kinase (pgk) CSB-YP808391LAS
CSB-EP808391LAS
CSB-BP808391LAS
CSB-MP808391LAS
CSB-EP808391LAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP808392BVW
CSB-EP808392BVW
CSB-BP808392BVW
CSB-MP808392BVW
CSB-EP808392BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenorhabdus nematophila Chaperone protein hscA (hscA), partial CSB-YP808393XBH
CSB-EP808393XBH
CSB-BP808393XBH
CSB-MP808393XBH
CSB-EP808393XBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae Bifunctional protein HldE (hldE) CSB-YP808394AXG
CSB-EP808394AXG
CSB-BP808394AXG
CSB-MP808394AXG
CSB-EP808394AXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma hominis 30S ribosomal protein S19 (rpsS) CSB-YP808395MWJ
CSB-EP808395MWJ
CSB-BP808395MWJ
CSB-MP808395MWJ
CSB-EP808395MWJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 6-phosphofructokinase (pfkA) CSB-YP808396HSY
CSB-EP808396HSY
CSB-BP808396HSY
CSB-MP808396HSY
CSB-EP808396HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsular polysaccharide phosphotransferase eps10H (eps10H) CSB-YP808397FNQ
CSB-EP808397FNQ
CSB-BP808397FNQ
CSB-MP808397FNQ
CSB-EP808397FNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c biogenesis ATP-binding export protein CcmA (ccmA) CSB-YP808398EZW
CSB-EP808398EZW
CSB-BP808398EZW
CSB-MP808398EZW
CSB-EP808398EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inorganic pyrophosphatase (ppa) CSB-YP808399DNW
CSB-EP808399DNW
CSB-BP808399DNW
CSB-MP808399DNW
CSB-EP808399DNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Aquaporin PIP2-4 (PIP2-4), partial CSB-YP808400OFG1
CSB-EP808400OFG1
CSB-BP808400OFG1
CSB-MP808400OFG1
CSB-EP808400OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calmodulin-binding transcription activator 3 (CMTA3), partial CSB-YP808401DOA
CSB-EP808401DOA
CSB-BP808401DOA
CSB-MP808401DOA
CSB-EP808401DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Lipoxygenase 2.2, chloroplastic (LOX2.2), partial CSB-YP808402HWQ
CSB-EP808402HWQ
CSB-BP808402HWQ
CSB-MP808402HWQ
CSB-EP808402HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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