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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana F-box/LRR-repeat protein At3g58930 (At3g58930) CSB-YP808423DOA
CSB-EP808423DOA
CSB-BP808423DOA
CSB-MP808423DOA
CSB-EP808423DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serine/threonine-protein kinase At5g01020 (At5g01020) CSB-YP808424DOA
CSB-EP808424DOA
CSB-BP808424DOA
CSB-MP808424DOA
CSB-EP808424DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH63 (BHLH63) CSB-YP808425DOA
CSB-EP808425DOA
CSB-BP808425DOA
CSB-MP808425DOA
CSB-EP808425DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DEAD-box ATP-dependent RNA helicase 10 (RH10) CSB-YP808426DOA
CSB-EP808426DOA
CSB-BP808426DOA
CSB-MP808426DOA
CSB-EP808426DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cationic amino acid transporter 3, mitochondrial (CAT3), partial CSB-YP808427DOA
CSB-EP808427DOA
CSB-BP808427DOA
CSB-MP808427DOA
CSB-EP808427DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable inorganic phosphate transporter 1-5 (PHT1-5), partial CSB-YP808428DOA
CSB-EP808428DOA
CSB-BP808428DOA
CSB-MP808428DOA
CSB-EP808428DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana B3 domain-containing protein REM14 (REM14) CSB-YP808429DOA
CSB-EP808429DOA
CSB-BP808429DOA
CSB-MP808429DOA
CSB-EP808429DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana KH domain-containing protein At3g08620 (At3g08620) CSB-YP808430DOA
CSB-EP808430DOA
CSB-BP808430DOA
CSB-MP808430DOA
CSB-EP808430DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Heat stress transcription factor A-3 (HSFA3) CSB-YP808431DOA
CSB-EP808431DOA
CSB-BP808431DOA
CSB-MP808431DOA
CSB-EP808431DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable threonine--tRNA ligase, cytoplasmic (At1g17960) CSB-YP808432DOA
CSB-EP808432DOA
CSB-BP808432DOA
CSB-MP808432DOA
CSB-EP808432DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein Brevis radix-like 4 (BRXL4) CSB-YP808433DOA
CSB-EP808433DOA
CSB-BP808433DOA
CSB-MP808433DOA
CSB-EP808433DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Xyloglucan galactosyltransferase KATAMARI1 homolog (Os03g0144800, LOC_Os03g05110), partial CSB-YP808434OFG
CSB-EP808434OFG
CSB-BP808434OFG
CSB-MP808434OFG
CSB-EP808434OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coffea arabica 3,7-dimethylxanthine N-methyltransferase (DXMT1) CSB-YP808435DVA
CSB-EP808435DVA
CSB-BP808435DVA
CSB-MP808435DVA
CSB-EP808435DVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Katanin p80 WD40 repeat-containing subunit B1 homolog 1 (At5g23430), partial CSB-YP808436DOA
CSB-EP808436DOA
CSB-BP808436DOA
CSB-MP808436DOA
CSB-EP808436DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH128 (BHLH128) CSB-YP808437DOA
CSB-EP808437DOA
CSB-BP808437DOA
CSB-MP808437DOA
CSB-EP808437DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At2g34460, chloroplastic (At2g34460) CSB-YP808438DOA
CSB-EP808438DOA
CSB-BP808438DOA
CSB-MP808438DOA
CSB-EP808438DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Malonate--CoA ligase (AAE13), partial CSB-YP808439DOA
CSB-EP808439DOA
CSB-BP808439DOA
CSB-MP808439DOA
CSB-EP808439DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 40S ribosomal protein Sa-2 (RPSaB) CSB-YP808440DOA
CSB-EP808440DOA
CSB-BP808440DOA
CSB-MP808440DOA
CSB-EP808440DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable receptor-like protein kinase At3g55450 (At3g55450) CSB-YP808441DOA
CSB-EP808441DOA
CSB-BP808441DOA
CSB-MP808441DOA
CSB-EP808441DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pachytene checkpoint protein 2 homolog (At4g24710) CSB-YP808442DOA
CSB-EP808442DOA
CSB-BP808442DOA
CSB-MP808442DOA
CSB-EP808442DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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