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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella suis biovar 1 50S ribosomal protein L17 (rplQ) CSB-YP808323BMQ
CSB-EP808323BMQ
CSB-BP808323BMQ
CSB-MP808323BMQ
CSB-EP808323BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Glutamate--tRNA ligase 2 (gltX2) CSB-YP808324BMQ
CSB-EP808324BMQ
CSB-BP808324BMQ
CSB-MP808324BMQ
CSB-EP808324BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP808325BMQ
CSB-EP808325BMQ
CSB-BP808325BMQ
CSB-MP808325BMQ
CSB-EP808325BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 UvrABC system protein A (uvrA), partial CSB-YP808326BMQ
CSB-EP808326BMQ
CSB-BP808326BMQ
CSB-MP808326BMQ
CSB-EP808326BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Flavoprotein WrbA (wrbA) CSB-YP808327BMQ
CSB-EP808327BMQ
CSB-BP808327BMQ
CSB-MP808327BMQ
CSB-EP808327BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP808328BMQ
CSB-EP808328BMQ
CSB-BP808328BMQ
CSB-MP808328BMQ
CSB-EP808328BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP808329BMQ
CSB-EP808329BMQ
CSB-BP808329BMQ
CSB-MP808329BMQ
CSB-EP808329BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP808330BMQ
CSB-EP808330BMQ
CSB-BP808330BMQ
CSB-MP808330BMQ
CSB-EP808330BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP808331BMQ
CSB-EP808331BMQ
CSB-BP808331BMQ
CSB-MP808331BMQ
CSB-EP808331BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 HTH-type transcriptional regulator BetI (betI) CSB-YP808332BMQ
CSB-EP808332BMQ
CSB-BP808332BMQ
CSB-MP808332BMQ
CSB-EP808332BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 UPF0301 protein BR0480/BS1330_I0481 (BR0480, BS1330_I0481) CSB-YP808333BMQ
CSB-EP808333BMQ
CSB-BP808333BMQ
CSB-MP808333BMQ
CSB-EP808333BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP808334BMQ
CSB-EP808334BMQ
CSB-BP808334BMQ
CSB-MP808334BMQ
CSB-EP808334BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Pantothenate synthetase (panC) CSB-YP808335BMQ
CSB-EP808335BMQ
CSB-BP808335BMQ
CSB-MP808335BMQ
CSB-EP808335BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Efflux pump membrane transporter BepE (bepE), partial CSB-YP808336BMQ
CSB-EP808336BMQ
CSB-BP808336BMQ
CSB-MP808336BMQ
CSB-EP808336BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 HTH-type transcriptional repressor BepR (bepR) CSB-YP808337BMQ
CSB-EP808337BMQ
CSB-BP808337BMQ
CSB-MP808337BMQ
CSB-EP808337BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Urease subunit alpha 1 (ureC1), partial CSB-YP808338BMQ
CSB-EP808338BMQ
CSB-BP808338BMQ
CSB-MP808338BMQ
CSB-EP808338BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Histidinol dehydrogenase (hisD) CSB-YP808339BMQ
CSB-EP808339BMQ
CSB-BP808339BMQ
CSB-MP808339BMQ
CSB-EP808339BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 [Protein-PII] uridylyltransferase (glnD), partial CSB-YP808340BMQ
CSB-EP808340BMQ
CSB-BP808340BMQ
CSB-MP808340BMQ
CSB-EP808340BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP808341BMQ
CSB-EP808341BMQ
CSB-BP808341BMQ
CSB-MP808341BMQ
CSB-EP808341BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Methionyl-tRNA formyltransferase (fmt) CSB-YP808342BGP
CSB-EP808342BGP
CSB-BP808342BGP
CSB-MP808342BGP
CSB-EP808342BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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