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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila melanogaster Protein Turandot X (TotX) CSB-YP808523DLU
CSB-EP808523DLU
CSB-BP808523DLU
CSB-MP808523DLU
CSB-EP808523DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Trithorax group protein osa (osa), partial CSB-YP808524DLU
CSB-EP808524DLU
CSB-BP808524DLU
CSB-MP808524DLU
CSB-EP808524DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein MEF2BNB homolog (CG32590) CSB-YP808525DLU
CSB-EP808525DLU
CSB-BP808525DLU
CSB-MP808525DLU
CSB-EP808525DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster cAMP-specific 3',5'-cyclic phosphodiesterase, isoform F (dnc), partial CSB-YP808526DLU
CSB-EP808526DLU
CSB-BP808526DLU
CSB-MP808526DLU
CSB-EP808526DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ras guanine nucleotide exchange factor J (gefJ), partial CSB-YP808527DKK
CSB-EP808527DKK
CSB-BP808527DKK
CSB-MP808527DKK
CSB-EP808527DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta Beta-1,3-glucan-binding protein 2 CSB-YP808528MPV
CSB-EP808528MPV
CSB-BP808528MPV
CSB-MP808528MPV
CSB-EP808528MPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma belcheri 60S ribosomal protein L24 (RPL24) CSB-YP808529BVX
CSB-EP808529BVX
CSB-BP808529BVX
CSB-MP808529BVX
CSB-EP808529BVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mnemiopsis leidyi Forkhead box protein G1 (FOXG1) CSB-YP808530MHAM
CSB-EP808530MHAM
CSB-BP808530MHAM
CSB-MP808530MHAM
CSB-EP808530MHAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane protease serine 6 (TMPRSS6), partial CSB-YP808531HU
CSB-EP808531HU
CSB-BP808531HU
CSB-MP808531HU
CSB-EP808531HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Calcium homeostasis modulator protein 1 (CALHM1), partial CSB-YP808532HU1
CSB-EP808532HU1
CSB-BP808532HU1
CSB-MP808532HU1
CSB-EP808532HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human WAP four-disulfide core domain protein 13 (WFDC13) CSB-YP808533HU
CSB-EP808533HU
CSB-BP808533HU
CSB-MP808533HU
CSB-EP808533HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Homeobox protein TGIF2LX (TGIF2LX) CSB-YP808534HU
CSB-BP808534HU
CSB-MP808534HU
CSB-EP808534HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein CREG2 (CREG2) CSB-YP808535HU
CSB-EP808535HU
CSB-BP808535HU
CSB-MP808535HU
CSB-EP808535HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative RUN domain-containing protein 2B (SNX29P1) CSB-YP808536HU
CSB-EP808536HU
CSB-BP808536HU
CSB-MP808536HU
CSB-EP808536HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane and TPR repeat-containing protein 1 (TMTC1), partial CSB-YP808537HU
CSB-EP808537HU
CSB-BP808537HU
CSB-MP808537HU
CSB-EP808537HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein hid-1 homolog (C17orf28) CSB-YP808538HU
CSB-EP808538HU
CSB-BP808538HU
CSB-MP808538HU
CSB-EP808538HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ras guanyl-releasing protein 3 (RASGRP3) CSB-YP808539HU
CSB-BP808539HU
CSB-MP808539HU
CSB-EP808539HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cyclic nucleotide-gated cation channel alpha-4 (CNGA4), partial CSB-YP808540HU
CSB-EP808540HU
CSB-BP808540HU
CSB-EP808540HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human LIX1-like protein (LIX1L) CSB-YP808541HU
CSB-EP808541HU
CSB-BP808541HU
CSB-MP808541HU
CSB-EP808541HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Dynein heavy chain 10, axonemal (DNAH10), partial CSB-YP808542HU
CSB-EP808542HU
CSB-BP808542HU
CSB-MP808542HU
CSB-EP808542HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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