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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Protein pmbA homolog (pmbA) CSB-YP809173BXE
CSB-EP809173BXE
CSB-BP809173BXE
CSB-MP809173BXE
CSB-EP809173BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Flagellar hook-basal body complex protein FliE (fliE) CSB-YP809174BXE
CSB-EP809174BXE
CSB-BP809174BXE
CSB-MP809174BXE
CSB-EP809174BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Undecaprenyl-diphosphatase (uppP), partial CSB-YP809175BXE
CSB-EP809175BXE
CSB-BP809175BXE
CSB-MP809175BXE
CSB-EP809175BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 30S ribosomal protein S21 (rpsU) CSB-YP809176BXE
CSB-EP809176BXE
CSB-BP809176BXE
CSB-MP809176BXE
CSB-EP809176BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP809177BXE
CSB-EP809177BXE
CSB-BP809177BXE
CSB-MP809177BXE
CSB-EP809177BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Primosomal protein 1 (dnaT) CSB-YP809178BXE
CSB-EP809178BXE
CSB-BP809178BXE
CSB-MP809178BXE
CSB-EP809178BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Phosphoenolpyruvate carboxykinase [GTP] (pckG), partial CSB-YP809179DST
CSB-EP809179DST
CSB-BP809179DST
CSB-MP809179DST
CSB-EP809179DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum 50S ribosomal protein L3 (rplC) CSB-YP809180DST
CSB-EP809180DST
CSB-BP809180DST
CSB-MP809180DST
CSB-EP809180DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum 50S ribosomal protein L14 (rplN) CSB-YP809181DST
CSB-EP809181DST
CSB-BP809181DST
CSB-MP809181DST
CSB-EP809181DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum 30S ribosomal protein S11 (rpsK) CSB-YP809182DST
CSB-EP809182DST
CSB-BP809182DST
CSB-MP809182DST
CSB-EP809182DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Single-stranded DNA-binding protein 2 (ssb2) CSB-YP809183DST
CSB-EP809183DST
CSB-BP809183DST
CSB-MP809183DST
CSB-EP809183DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum DNA mismatch repair protein mutL (mutL), partial CSB-YP809184DST
CSB-EP809184DST
CSB-BP809184DST
CSB-MP809184DST
CSB-EP809184DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum 30S ribosomal protein S21 (rpsU) CSB-YP809185DST
CSB-EP809185DST
CSB-BP809185DST
CSB-MP809185DST
CSB-EP809185DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Lipoprotein signal peptidase (lspA), partial CSB-YP809186DST1
CSB-EP809186DST1
CSB-BP809186DST1
CSB-MP809186DST1
CSB-EP809186DST1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum GTPase Der (der) CSB-YP809187DST
CSB-EP809187DST
CSB-BP809187DST
CSB-MP809187DST
CSB-EP809187DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Phosphoribosylamine--glycine ligase (purD) CSB-YP809188DST
CSB-EP809188DST
CSB-BP809188DST
CSB-MP809188DST
CSB-EP809188DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP809189DST
CSB-EP809189DST
CSB-BP809189DST
CSB-MP809189DST
CSB-EP809189DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Serine hydroxymethyltransferase (glyA) CSB-YP809190DST
CSB-EP809190DST
CSB-BP809190DST
CSB-MP809190DST
CSB-EP809190DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum DNA mismatch repair protein MutS (mutS), partial CSB-YP809191DST
CSB-EP809191DST
CSB-BP809191DST
CSB-MP809191DST
CSB-EP809191DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Chaperone protein DnaJ (dnaJ) CSB-YP809192DST
CSB-EP809192DST
CSB-BP809192DST
CSB-MP809192DST
CSB-EP809192DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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