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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Regulatory protein luxO (luxO) CSB-YP809411VFI
CSB-EP809411VFI
CSB-BP809411VFI
CSB-MP809411VFI
CSB-EP809411VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA dimethylallyltransferase (miaA) CSB-YP809412VFI
CSB-EP809412VFI
CSB-BP809412VFI
CSB-MP809412VFI
CSB-EP809412VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP809413TDQ
CSB-EP809413TDQ
CSB-BP809413TDQ
CSB-MP809413TDQ
CSB-EP809413TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Aspartate--tRNA ligase (aspS), partial CSB-YP809414SMP
CSB-EP809414SMP
CSB-BP809414SMP
CSB-MP809414SMP
CSB-EP809414SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae tRNA pseudouridine synthase A (truA) CSB-YP809415SMP
CSB-EP809415SMP
CSB-BP809415SMP
CSB-MP809415SMP
CSB-EP809415SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae tRNA pseudouridine synthase B (truB) CSB-YP809416SMP
CSB-EP809416SMP
CSB-BP809416SMP
CSB-MP809416SMP
CSB-EP809416SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Chaperone protein DnaK (dnaK), partial CSB-YP809417SMP
CSB-EP809417SMP
CSB-BP809417SMP
CSB-MP809417SMP
CSB-EP809417SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 50S ribosomal protein L5 (rplE) CSB-YP809418SMP
CSB-EP809418SMP
CSB-BP809418SMP
CSB-MP809418SMP
CSB-EP809418SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP809419SMP
CSB-EP809419SMP
CSB-BP809419SMP
CSB-MP809419SMP
CSB-EP809419SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP809420FMR
CSB-EP809420FMR
CSB-BP809420FMR
CSB-MP809420FMR
CSB-EP809420FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-diphosphatase (uppP), partial CSB-YP809421FMR1
CSB-EP809421FMR1
CSB-BP809421FMR1
CSB-MP809421FMR1
CSB-EP809421FMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA dimethylallyltransferase (miaA) CSB-YP809422SMI
CSB-EP809422SMI
CSB-BP809422SMI
CSB-MP809422SMI
CSB-EP809422SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP809423SMH
CSB-EP809423SMH
CSB-BP809423SMH
CSB-MP809423SMH
CSB-EP809423SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP809424SGA
CSB-EP809424SGA
CSB-BP809424SGA
CSB-MP809424SGA
CSB-EP809424SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Alanine dehydrogenase (ald) CSB-YP809425OAB
CSB-EP809425OAB
CSB-BP809425OAB
CSB-MP809425OAB
CSB-EP809425OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Porphobilinogen deaminase (hemC) CSB-YP809426OAB
CSB-EP809426OAB
CSB-BP809426OAB
CSB-MP809426OAB
CSB-EP809426OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Protein grpE (grpE) CSB-YP809427OAB
CSB-EP809427OAB
CSB-BP809427OAB
CSB-MP809427OAB
CSB-EP809427OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP809428OAB
CSB-EP809428OAB
CSB-BP809428OAB
CSB-MP809428OAB
CSB-EP809428OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Protein RecA (recA) CSB-YP809429OAB
CSB-EP809429OAB
CSB-BP809429OAB
CSB-MP809429OAB
CSB-EP809429OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Carbamoyl-phosphate synthase small chain (carA) CSB-YP809430OAB
CSB-EP809430OAB
CSB-BP809430OAB
CSB-MP809430OAB
CSB-EP809430OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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