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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Uncharacterized metalloprotease BUsg_310 (BUsg_310), partial CSB-YP809153BXE1
CSB-EP809153BXE1
CSB-BP809153BXE1
CSB-MP809153BXE1
CSB-EP809153BXE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP809154BXE
CSB-EP809154BXE
CSB-BP809154BXE
CSB-MP809154BXE
CSB-EP809154BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Biotin synthase (bioB) CSB-YP809155BXE
CSB-EP809155BXE
CSB-BP809155BXE
CSB-MP809155BXE
CSB-EP809155BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum DNA topoisomerase 1 (topA), partial CSB-YP809156BXE
CSB-EP809156BXE
CSB-BP809156BXE
CSB-MP809156BXE
CSB-EP809156BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP809157BXE
CSB-EP809157BXE
CSB-BP809157BXE
CSB-MP809157BXE
CSB-EP809157BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Signal peptidase I (lepB), partial CSB-YP809158BXE
CSB-EP809158BXE
CSB-BP809158BXE
CSB-MP809158BXE
CSB-EP809158BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum SsrA-binding protein (smpB) CSB-YP809159BXE
CSB-EP809159BXE
CSB-BP809159BXE
CSB-MP809159BXE
CSB-EP809159BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Uncharacterized protein BUsg_231 (BUsg_231), partial CSB-YP809160BXE
CSB-EP809160BXE
CSB-BP809160BXE
CSB-MP809160BXE
CSB-EP809160BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 30S ribosomal protein S2 (rpsB) CSB-YP809161BXE
CSB-EP809161BXE
CSB-BP809161BXE
CSB-MP809161BXE
CSB-EP809161BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Protein translocase subunit SecA (secA), partial CSB-YP809162BXE
CSB-EP809162BXE
CSB-BP809162BXE
CSB-MP809162BXE
CSB-EP809162BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Bifunctional aspartokinase/homoserine dehydrogenase (thrA), partial CSB-YP809163BXE
CSB-EP809163BXE
CSB-BP809163BXE
CSB-MP809163BXE
CSB-EP809163BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Small protein A homolog (smpA) CSB-YP809164BXE
CSB-EP809164BXE
CSB-BP809164BXE
CSB-MP809164BXE
CSB-EP809164BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Acetate kinase (ackA) CSB-YP809165BXE
CSB-EP809165BXE
CSB-BP809165BXE
CSB-MP809165BXE
CSB-EP809165BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Peptide chain release factor 1 (prfA) CSB-YP809166BXE
CSB-EP809166BXE
CSB-BP809166BXE
CSB-MP809166BXE
CSB-EP809166BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum NADH-quinone oxidoreductase subunit L (nuoL), partial CSB-YP809167BXE
CSB-EP809167BXE
CSB-BP809167BXE
CSB-MP809167BXE
CSB-EP809167BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP809168BXE
CSB-EP809168BXE
CSB-BP809168BXE
CSB-MP809168BXE
CSB-EP809168BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Isoleucine--tRNA ligase (ileS), partial CSB-YP809169BXE
CSB-EP809169BXE
CSB-BP809169BXE
CSB-MP809169BXE
CSB-EP809169BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 50S ribosomal protein L25 (rplY) CSB-YP809170BXE
CSB-EP809170BXE
CSB-BP809170BXE
CSB-MP809170BXE
CSB-EP809170BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Tyrosine--tRNA ligase (tyrS) CSB-YP809171BXE
CSB-EP809171BXE
CSB-BP809171BXE
CSB-MP809171BXE
CSB-EP809171BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Electron transport complex protein RnfB (rnfB) CSB-YP809172BXE
CSB-EP809172BXE
CSB-BP809172BXE
CSB-MP809172BXE
CSB-EP809172BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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