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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Synechococcus sp. Tryptophan synthase alpha chain (trpA) CSB-YP809233FQA
CSB-EP809233FQA
CSB-BP809233FQA
CSB-MP809233FQA
CSB-EP809233FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein pXO1-01/BXA0003/GBAA_pXO1_0003 (pXO1-01, BXA0003, GBAA_pXO1_0003) CSB-YP809234BQE
CSB-EP809234BQE
CSB-BP809234BQE
CSB-MP809234BQE
CSB-EP809234BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Taurine import ATP-binding protein TauB (tauB) CSB-YP809235FFZ
CSB-EP809235FFZ
CSB-BP809235FFZ
CSB-MP809235FFZ
CSB-EP809235FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-recycling factor (frr) CSB-YP809236PCE
CSB-EP809236PCE
CSB-BP809236PCE
CSB-MP809236PCE
CSB-EP809236PCE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI) CSB-YP809237TBAS
CSB-EP809237TBAS
CSB-BP809237TBAS
CSB-MP809237TBAS
CSB-EP809237TBAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein grpE (grpE) CSB-YP809238ABI
CSB-EP809238ABI
CSB-BP809238ABI
CSB-MP809238ABI
CSB-EP809238ABI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial (KAS) CSB-YP809239DOA
CSB-EP809239DOA
CSB-BP809239DOA
CSB-MP809239DOA
CSB-EP809239DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable galacturonosyltransferase 15 (GAUT15), partial CSB-YP809240DOA
CSB-EP809240DOA
CSB-BP809240DOA
CSB-MP809240DOA
CSB-EP809240DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable cation transporter HKT9 (HKT9), partial CSB-YP809241OFG
CSB-EP809241OFG
CSB-BP809241OFG
CSB-MP809241OFG
CSB-EP809241OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4 (LPAT4), partial CSB-YP809242DOA1
CSB-EP809242DOA1
CSB-BP809242DOA1
CSB-MP809242DOA1
CSB-EP809242DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Sorting nexin 2A (SNX2A), partial CSB-YP809243DOA
CSB-EP809243DOA
CSB-BP809243DOA
CSB-MP809243DOA
CSB-EP809243DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum DNA-directed RNA polymerase 2B, chloroplastic/mitochondrial (RPOT2-TOM), partial CSB-YP809244NHE
CSB-EP809244NHE
CSB-BP809244NHE
CSB-MP809244NHE
CSB-EP809244NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Splicing factor U2af large subunit B (U2AF65B), partial CSB-YP809245DOA
CSB-EP809245DOA
CSB-BP809245DOA
CSB-MP809245DOA
CSB-EP809245DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana AP-4 complex subunit epsilon (At1g31730), partial CSB-YP809246DOA
CSB-EP809246DOA
CSB-BP809246DOA
CSB-MP809246DOA
CSB-EP809246DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-1,3-galactosyltransferase 15 (B3GALT15), partial CSB-YP809247DOA
CSB-EP809247DOA
CSB-BP809247DOA
CSB-MP809247DOA
CSB-EP809247DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Telomere repeat-binding protein 1 (TRP1), partial CSB-YP809248DOA
CSB-EP809248DOA
CSB-BP809248DOA
CSB-MP809248DOA
CSB-EP809248DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger CCCH domain-containing protein 24 (At2g28450), partial CSB-YP809249DOA
CSB-EP809249DOA
CSB-BP809249DOA
CSB-MP809249DOA
CSB-EP809249DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable methyltransferase PMT26 (At5g64030), partial CSB-YP809250DOA
CSB-EP809250DOA
CSB-BP809250DOA
CSB-MP809250DOA
CSB-EP809250DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable cyclic nucleotide-gated ion channel 17 (CNGC17), partial CSB-YP809251DOA1
CSB-EP809251DOA1
CSB-BP809251DOA1
CSB-MP809251DOA1
CSB-EP809251DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nudix hydrolase 3 (NUDT3), partial CSB-YP809252DOA
CSB-EP809252DOA
CSB-BP809252DOA
CSB-MP809252DOA
CSB-EP809252DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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