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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus epidermidis Ribonuclease P protein component (rnpA) CSB-YP809311SLD
CSB-EP809311SLD
CSB-BP809311SLD
CSB-MP809311SLD
CSB-EP809311SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Alkyl hydroperoxide reductase subunit C (ahpC) CSB-YP809312SLD
CSB-EP809312SLD
CSB-BP809312SLD
CSB-MP809312SLD
CSB-EP809312SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Protein translocase subunit SecA 2 (secA2), partial CSB-YP809313SLD
CSB-EP809313SLD
CSB-BP809313SLD
CSB-MP809313SLD
CSB-EP809313SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis L-lactate dehydrogenase (ldh) CSB-YP809314SLD
CSB-EP809314SLD
CSB-BP809314SLD
CSB-MP809314SLD
CSB-EP809314SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP809315SLD
CSB-EP809315SLD
CSB-BP809315SLD
CSB-MP809315SLD
CSB-EP809315SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Phosphoglucomutase (pgcA), partial CSB-YP809316SLD
CSB-EP809316SLD
CSB-BP809316SLD
CSB-MP809316SLD
CSB-EP809316SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Oxygen regulatory protein nreC (nreC) CSB-YP809317SLD
CSB-EP809317SLD
CSB-BP809317SLD
CSB-MP809317SLD
CSB-EP809317SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Membrane-associated protein TcaA (tcaA), partial CSB-YP809318SLD
CSB-EP809318SLD
CSB-BP809318SLD
CSB-MP809318SLD
CSB-EP809318SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP809319SLD
CSB-EP809319SLD
CSB-BP809319SLD
CSB-MP809319SLD
CSB-EP809319SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis NAD-dependent protein deacetylase (cobB) CSB-YP809320SLD
CSB-EP809320SLD
CSB-BP809320SLD
CSB-MP809320SLD
CSB-EP809320SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Deoxyribose-phosphate aldolase (deoC) CSB-YP809321SLD
CSB-EP809321SLD
CSB-BP809321SLD
CSB-MP809321SLD
CSB-EP809321SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Low molecular weight protein-tyrosine-phosphatase ptpB (ptpB) CSB-YP809322SLD
CSB-EP809322SLD
CSB-BP809322SLD
CSB-MP809322SLD
CSB-EP809322SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Single-stranded DNA-binding protein (ssb) CSB-YP809323SLD
CSB-EP809323SLD
CSB-BP809323SLD
CSB-MP809323SLD
CSB-EP809323SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Alanine racemase (alr) CSB-YP809324SLD
CSB-EP809324SLD
CSB-BP809324SLD
CSB-MP809324SLD
CSB-EP809324SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable manganese-dependent inorganic pyrophosphatase (ppaC) CSB-YP809325SLD
CSB-EP809325SLD
CSB-BP809325SLD
CSB-MP809325SLD
CSB-EP809325SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Low molecular weight protein-tyrosine-phosphatase ptpA (ptpA) CSB-YP809326SLD
CSB-EP809326SLD
CSB-BP809326SLD
CSB-MP809326SLD
CSB-EP809326SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Signal transduction protein TRAP (traP) CSB-YP809327SLD
CSB-EP809327SLD
CSB-BP809327SLD
CSB-MP809327SLD
CSB-EP809327SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Uncharacterized peptidase SE_1383 (SE_1383) CSB-YP809328SLD
CSB-EP809328SLD
CSB-BP809328SLD
CSB-MP809328SLD
CSB-EP809328SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Trigger factor (tig) CSB-YP809329SLD
CSB-EP809329SLD
CSB-BP809329SLD
CSB-MP809329SLD
CSB-EP809329SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP809330SLD
CSB-EP809330SLD
CSB-BP809330SLD
CSB-MP809330SLD
CSB-EP809330SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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