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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O9:H4 ATP synthase subunit b (atpF), partial CSB-YP002358EJF1
CSB-EP002358EJF1
CSB-BP002358EJF1
CSB-MP002358EJF1
CSB-EP002358EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 tRNA dimethylallyltransferase (miaA) CSB-YP024529EJF
CSB-EP024529EJF
CSB-BP024529EJF
CSB-MP024529EJF
CSB-EP024529EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Nucleoside diphosphate kinase (ndk) CSB-YP015889ILH
CSB-EP015889ILH
CSB-BP015889ILH
CSB-MP015889ILH
CSB-EP015889ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri ATP synthase subunit b (atpF), partial CSB-YP002358CWS1
CSB-EP002358CWS1
CSB-BP002358CWS1
CSB-MP002358CWS1
CSB-EP002358CWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Serine hydroxymethyltransferase (glyA) CSB-YP021274CWS
CSB-EP021274CWS
CSB-BP021274CWS
CSB-MP021274CWS
CSB-EP021274CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri tRNA pseudouridine synthase A (truA) CSB-YP019086CWS
CSB-EP019086CWS
CSB-BP019086CWS
CSB-MP019086CWS
CSB-EP019086CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Urocanate hydratase (hutU), partial CSB-YP025677CWS
CSB-EP025677CWS
CSB-BP025677CWS
CSB-MP025677CWS
CSB-EP025677CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Oligoribonuclease (orn) CSB-YP019586CWS
CSB-EP019586CWS
CSB-BP019586CWS
CSB-MP019586CWS
CSB-EP019586CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Thymidylate synthase (thyA) CSB-YP025393CWS
CSB-EP025393CWS
CSB-BP025393CWS
CSB-MP025393CWS
CSB-EP025393CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146FMN
CSB-EP019146FMN
CSB-BP019146FMN
CSB-MP019146FMN
CSB-EP019146FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Ribosome-recycling factor (frr) CSB-YP014941FMN
CSB-EP014941FMN
CSB-BP014941FMN
CSB-MP014941FMN
CSB-EP014941FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Elongation factor Ts (tsf) CSB-YP025124FMN
CSB-EP025124FMN
CSB-BP025124FMN
CSB-MP025124FMN
CSB-EP025124FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis Inosine triphosphate pyrophosphatase (GL50803_7511) CSB-YP011907GFY
CSB-EP011907GFY
CSB-BP011907GFY
CSB-MP011907GFY
CSB-EP011907GFY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca hecki Agouti-signaling protein (ASIP) CSB-YP002212MMY
CSB-EP002212MMY
CSB-BP002212MMY
CSB-MP002212MMY
CSB-EP002212MMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avahi unicolor NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP015080AVI1
CSB-EP015080AVI1
CSB-BP015080AVI1
CSB-MP015080AVI1
CSB-EP015080AVI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Glutamate receptor-interacting protein 2 (grip2), partial CSB-YP009922XBE
CSB-EP009922XBE
CSB-BP009922XBE
CSB-MP009922XBE
CSB-EP009922XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Lysine-specific demethylase 8 (jmjd5) CSB-YP011953DIL
CSB-EP011953DIL
CSB-BP011953DIL
CSB-MP011953DIL
CSB-EP011953DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Malate dehydrogenase (mdh) CSB-YP013623AUY
CSB-EP013623AUY
CSB-BP013623AUY
CSB-MP013623AUY
CSB-EP013623AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Elongation factor Ts (tsf) CSB-YP025124RMR
CSB-EP025124RMR
CSB-BP025124RMR
CSB-MP025124RMR
CSB-EP025124RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis GTPase Era (era) CSB-YP007759RMR
CSB-EP007759RMR
CSB-BP007759RMR
CSB-MP007759RMR
CSB-EP007759RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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