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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus tropicalis Cyclin-dependent kinase 12 (cdk12), partial CSB-YP005981XBF
CSB-EP005981XBF
CSB-BP005981XBF
CSB-MP005981XBF
CSB-EP005981XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Rho GTPase-activating protein 26 (arhgap26), partial CSB-YP002027XBF
CSB-EP002027XBF
CSB-BP002027XBF
CSB-MP002027XBF
CSB-EP002027XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Pancreatic progenitor cell differentiation and proliferation factor (ppdpf) CSB-YP003681SWI
CSB-EP003681SWI
CSB-BP003681SWI
CSB-MP003681SWI
CSB-EP003681SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila pseudoobscura pseudoobscura Eukaryotic translation initiation factor 3 subunit H (eIF-3p40) CSB-YP007537DME
CSB-EP007537DME
CSB-BP007537DME
CSB-MP007537DME
CSB-EP007537DME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila pseudoobscura pseudoobscura Ubiquitin-conjugating enzyme E2 S (GA22810) CSB-YP025477DME
CSB-EP025477DME
CSB-BP025477DME
CSB-MP025477DME
CSB-EP025477DME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila pseudoobscura pseudoobscura Eukaryotic translation initiation factor 3 subunit B (eIF3-S9), partial CSB-YP007530DME
CSB-EP007530DME
CSB-BP007530DME
CSB-MP007530DME
CSB-EP007530DME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F ATP synthase subunit a (atpB), partial CSB-YP015070FNE1
CSB-EP015070FNE1
CSB-BP015070FNE1
CSB-MP015070FNE1
CSB-EP015070FNE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F tRNA pseudouridine synthase A (truA) CSB-YP019086FNE
CSB-EP019086FNE
CSB-BP019086FNE
CSB-MP019086FNE
CSB-EP019086FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Triosephosphate isomerase (tpiA) CSB-YP024102GFN
CSB-EP024102GFN
CSB-BP024102GFN
CSB-MP024102GFN
CSB-EP024102GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis ATP synthase subunit a (atpB), partial CSB-YP015070GFN1
CSB-EP015070GFN1
CSB-BP015070GFN1
CSB-MP015070GFN1
CSB-EP015070GFN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033GFN
CSB-EP018033GFN
CSB-BP018033GFN
CSB-MP018033GFN
CSB-EP018033GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Ferrochelatase (hemH) CSB-YP008579GFN
CSB-EP008579GFN
CSB-BP008579GFN
CSB-MP008579GFN
CSB-EP008579GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Nucleoside diphosphate kinase (ndk) CSB-YP015889AWI
CSB-EP015889AWI
CSB-BP015889AWI
CSB-MP015889AWI
CSB-EP015889AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans ATP synthase subunit alpha (atpA), partial CSB-YP002344AWI
CSB-EP002344AWI
CSB-BP002344AWI
CSB-MP002344AWI
CSB-EP002344AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Transaldolase (tal) CSB-YP023112VFC
CSB-EP023112VFC
CSB-BP023112VFC
CSB-MP023112VFC
CSB-EP023112VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Lipoyl synthase (lipA) CSB-YP012927SWK
CSB-EP012927SWK
CSB-BP012927SWK
CSB-MP012927SWK
CSB-EP012927SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Phosphoserine aminotransferase (serC) CSB-YP018838SWK
CSB-EP018838SWK
CSB-BP018838SWK
CSB-MP018838SWK
CSB-EP018838SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona GTPase Era (era) CSB-YP007759SWK
CSB-EP007759SWK
CSB-BP007759SWK
CSB-MP007759SWK
CSB-EP007759SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451SWK
CSB-EP015451SWK
CSB-BP015451SWK
CSB-MP015451SWK
CSB-EP015451SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033SWK
CSB-EP018033SWK
CSB-BP018033SWK
CSB-MP018033SWK
CSB-EP018033SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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