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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bothrops erythromelas NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079BUV1
CSB-EP015079BUV1
CSB-BP015079BUV1
CSB-MP015079BUV1
CSB-EP015079BUV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus oreganus concolor NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079DYF1
CSB-EP015079DYF1
CSB-BP015079DYF1
CSB-MP015079DYF1
CSB-EP015079DYF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porthidium hyoprora NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079EYB1
CSB-EP015079EYB1
CSB-BP015079EYB1
CSB-MP015079EYB1
CSB-EP015079EYB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptelytrops cantori NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079DYJ1
CSB-EP015079DYJ1
CSB-BP015079DYJ1
CSB-MP015079DYJ1
CSB-EP015079DYJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratotherium simum NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076DRG
CSB-EP015076DRG
CSB-BP015076DRG
CSB-MP015076DRG
CSB-EP015076DRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tinamus major Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073TOL1
CSB-EP015073TOL1
CSB-BP015073TOL1
CSB-MP015073TOL1
CSB-EP015073TOL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinidia deliciosa Delta-1-pyrroline-5-carboxylate synthase, partial CSB-YP001564AXF
CSB-EP001564AXF
CSB-BP001564AXF
CSB-MP001564AXF
CSB-EP001564AXF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ricinus communis Acyl-CoA-binding protein CSB-YP006519RMM
CSB-EP006519RMM
CSB-BP006519RMM
CSB-MP006519RMM
CSB-EP006519RMM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica campestris GTP-binding protein SAR1B (SAR1B) CSB-YP020703BWB
CSB-EP020703BWB
CSB-BP020703BWB
CSB-MP020703BWB
CSB-EP020703BWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana G-protein coupled receptor 1 (GCR1), partial CSB-YP009728DOA1
CSB-EP009728DOA1
CSB-BP009728DOA1
CSB-MP009728DOA1
CSB-EP009728DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GTP-binding protein SAR1A (SAR1A) CSB-YP020702DOA
CSB-EP020702DOA
CSB-BP020702DOA
CSB-MP020702DOA
CSB-EP020702DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium acetobutylicum ATP synthase subunit a (atpB), partial CSB-YP015070DUB1
CSB-EP015070DUB1
CSB-BP015070DUB1
CSB-MP015070DUB1
CSB-EP015070DUB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus ATP synthase subunit b (atpF), partial CSB-YP002358RLC1
CSB-EP002358RLC1
CSB-BP002358RLC1
CSB-MP002358RLC1
CSB-EP002358RLC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia prowazekii Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917RMY
CSB-EP022917RMY
CSB-BP022917RMY
CSB-MP022917RMY
CSB-EP022917RMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Nicotinate-nucleotide pyrophosphorylase [carboxylating] (nadC) CSB-YP019137MVZ
CSB-EP019137MVZ
CSB-BP019137MVZ
CSB-MP019137MVZ
CSB-EP019137MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824MVZ
CSB-EP005824MVZ
CSB-BP005824MVZ
CSB-MP005824MVZ
CSB-EP005824MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Malate dehydrogenase (mdh) CSB-YP013623DOC
CSB-EP013623DOC
CSB-BP013623DOC
CSB-MP013623DOC
CSB-EP013623DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Peptide deformylase (def) CSB-YP017707DUC
CSB-EP017707DUC
CSB-BP017707DUC
CSB-MP017707DUC
CSB-EP017707DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Phospholipase D1 (PLD1), partial CSB-YP018144DXU
CSB-EP018144DXU
CSB-BP018144DXU
CSB-MP018144DXU
CSB-EP018144DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Glycogenin-1 (Gyg1) CSB-YP010070RA
CSB-EP010070RA
CSB-BP010070RA
CSB-MP010070RA
CSB-EP010070RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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