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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Zunongwangia profunda Ribonuclease 3 (rnc) CSB-YP019809ZBI
CSB-EP019809ZBI
CSB-BP019809ZBI
CSB-MP019809ZBI
CSB-EP019809ZBI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanohalobium evestigatum FAD synthase (ribL) CSB-YP008710MSF
CSB-EP008710MSF
CSB-BP008710MSF
CSB-MP008710MSF
CSB-EP008710MSF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera Methylthioribose-1-phosphate isomerase (VIT_11s0016g00830) CSB-YP014799VFQ
CSB-EP014799VFQ
CSB-BP014799VFQ
CSB-MP014799VFQ
CSB-EP014799VFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein arginine N-methyltransferase 2 (prmt2) CSB-YP018732XBE
CSB-EP018732XBE
CSB-BP018732XBE
CSB-MP018732XBE
CSB-EP018732XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Vertnin (VRTN), partial CSB-YP003138BO
CSB-EP003138BO
CSB-BP003138BO
CSB-MP003138BO
CSB-EP003138BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Ubiquitin carboxyl-terminal hydrolase 37 (USP37), partial CSB-YP025728CH
CSB-EP025728CH
CSB-BP025728CH
CSB-MP025728CH
CSB-EP025728CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Protein MMS22-like (MMS22L), partial CSB-YP004078CH
CSB-EP004078CH
CSB-BP004078CH
CSB-MP004078CH
CSB-EP004078CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colletotrichum graminicola Inosine triphosphate pyrophosphatase (GLRG_03307) CSB-YP011907DVF
CSB-EP011907DVF
CSB-BP011907DVF
CSB-MP011907DVF
CSB-EP011907DVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Neurobeachin-like protein 2 (nbeal2), partial CSB-YP015484DIL
CSB-EP015484DIL
CSB-BP015484DIL
CSB-MP015484DIL
CSB-EP015484DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serine/threonine-protein kinase 10 (Stk10), partial CSB-YP022832RA
CSB-EP022832RA
CSB-BP022832RA
CSB-MP022832RA
CSB-EP022832RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Receptor-type tyrosine-protein phosphatase O (Ptpro), partial CSB-YP019061MO
CSB-EP019061MO
CSB-BP019061MO
CSB-MP019061MO
CSB-EP019061MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Rap guanine nucleotide exchange factor 2 (Rapgef2), partial CSB-YP019330RA
CSB-EP019330RA
CSB-BP019330RA
CSB-MP019330RA
CSB-EP019330RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine AP2-associated protein kinase 1 (AAK1), partial CSB-YP001020BO
CSB-EP001020BO
CSB-BP001020BO
CSB-MP001020BO
CSB-EP001020BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine FUN14 domain-containing protein 1 (FUNDC1), partial CSB-YP009066BO1
CSB-EP009066BO1
CSB-BP009066BO1
CSB-MP009066BO1
CSB-EP009066BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Muscleblind-like protein 2 (Mbnl2) CSB-YP013544RA
CSB-EP013544RA
CSB-BP013544RA
CSB-MP013544RA
CSB-EP013544RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Transmembrane protein 237 (tmem237) CSB-YP001641XBF
CSB-EP001641XBF
CSB-BP001641XBF
CSB-MP001641XBF
CSB-EP001641XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Syntaxin-12 (Stx12), partial CSB-YP022890RA1
CSB-EP022890RA1
CSB-BP022890RA1
CSB-MP022890RA1
CSB-EP022890RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum 60S acidic ribosomal protein P2 (MAL3P3.19) CSB-YP020342EWP
CSB-EP020342EWP
CSB-BP020342EWP
CSB-MP020342EWP
CSB-EP020342EWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eukaryotic translation initiation factor 3 subunit H (eif-3.H) CSB-YP007537CXY
CSB-EP007537CXY
CSB-BP007537CXY
CSB-MP007537CXY
CSB-EP007537CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemonchus contortus 60S ribosomal protein L9 CSB-YP020326HSW
CSB-EP020326HSW
CSB-BP020326HSW
CSB-MP020326HSW
CSB-EP020326HSW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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