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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pyrococcus horikoshii Xaa-Pro dipeptidase (pepQ) CSB-YP017784FHX
CSB-EP017784FHX
CSB-BP017784FHX
CSB-MP017784FHX
CSB-EP017784FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Protein archease (PH1536) CSB-YP026349FHX
CSB-BP026349FHX
CSB-MP026349FHX
CSB-EP026349FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Elongation factor 2 (fusA), partial CSB-YP007434FHX
CSB-EP007434FHX
CSB-BP007434FHX
CSB-MP007434FHX
CSB-EP007434FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Superoxide dismutase [Cu-Zn] (SOD1) CSB-YP022397CZF
CSB-EP022397CZF
CSB-BP022397CZF
CSB-MP022397CZF
CSB-EP022397CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans 60S ribosomal protein L13 (RPL13) CSB-YP020129CZD
CSB-EP020129CZD
CSB-BP020129CZD
CSB-MP020129CZD
CSB-EP020129CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica 40S ribosomal protein S4 (RPS4) CSB-YP020458YAP
CSB-EP020458YAP
CSB-BP020458YAP
CSB-MP020458YAP
CSB-EP020458YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha (cwp1) CSB-YP008777SXV
CSB-EP008777SXV
CSB-BP008777SXV
CSB-MP008777SXV
CSB-EP008777SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Dolichyl-phosphate beta-glucosyltransferase (alg5), partial CSB-YP001602SXV1
CSB-EP001602SXV1
CSB-BP001602SXV1
CSB-MP001602SXV1
CSB-EP001602SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Exosome complex component rrp42 (rrp42) CSB-YP007895SXV
CSB-EP007895SXV
CSB-BP007895SXV
CSB-MP007895SXV
CSB-EP007895SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymnaea stagnalis Nitric oxide synthase (NOS), partial CSB-YP015946LRQ
CSB-EP015946LRQ
CSB-BP015946LRQ
CSB-MP015946LRQ
CSB-EP015946LRQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Flotillin-1 (Flo) CSB-YP008727DLU
CSB-EP008727DLU
CSB-BP008727DLU
CSB-MP008727DLU
CSB-EP008727DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cercocebus atys C-X-C chemokine receptor type 4 (CXCR4), partial CSB-YP006254DRI1
CSB-EP006254DRI1
CSB-BP006254DRI1
CSB-MP006254DRI1
CSB-EP006254DRI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macropus eugenii Sperm surface protein Sp17 (SPA17) CSB-YP022451MPE
CSB-EP022451MPE
CSB-BP022451MPE
CSB-MP022451MPE
CSB-EP022451MPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anas falcata NADH-ubiquinone oxidoreductase chain 2 (MT-ND2), partial CSB-YP015077BZA
CSB-EP015077BZA
CSB-BP015077BZA
CSB-MP015077BZA
CSB-EP015077BZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sarcophyton glaucum NADH-ubiquinone oxidoreductase chain 4L (ND4L), partial CSB-YP015080SXJ1
CSB-EP015080SXJ1
CSB-BP015080SXJ1
CSB-MP015080SXJ1
CSB-EP015080SXJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myxine glutinosa Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074MWW1
CSB-EP015074MWW1
CSB-BP015074MWW1
CSB-MP015074MWW1
CSB-EP015074MWW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea mariana 60S ribosomal protein L13a (RPL13A) CSB-YP020130EVM
CSB-EP020130EVM
CSB-BP020130EVM
CSB-MP020130EVM
CSB-EP020130EVM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Anaphase-promoting complex subunit 4 (APC4), partial CSB-YP001699DOA
CSB-EP001699DOA
CSB-BP001699DOA
CSB-MP001699DOA
CSB-EP001699DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytoplasmic tRNA 2-thiolation protein 2 (CTU2) CSB-YP003313DOA
CSB-EP003313DOA
CSB-BP003313DOA
CSB-MP003313DOA
CSB-EP003313DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cicer arietinum 40S ribosomal protein SA (RAP40) CSB-YP020485DTI
CSB-EP020485DTI
CSB-BP020485DTI
CSB-MP020485DTI
CSB-EP020485DTI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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