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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Casuarius bennetti Cytochrome b (MT-CYB), partial CSB-YP015075DQL
CSB-EP015075DQL
CSB-BP015075DQL
CSB-MP015075DQL
CSB-EP015075DQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhea americana Cytochrome b (MT-CYB), partial CSB-YP015075RKB
CSB-EP015075RKB
CSB-BP015075RKB
CSB-MP015075RKB
CSB-EP015075RKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tinamus major Cytochrome c oxidase subunit 1 (MT-CO1), partial CSB-YP015072TOL
CSB-EP015072TOL
CSB-BP015072TOL
CSB-MP015072TOL
CSB-EP015072TOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothriechis lateralis NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079BUO1
CSB-EP015079BUO1
CSB-BP015079BUO1
CSB-MP015079BUO1
CSB-EP015079BUO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cerrophidion godmani NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079DRM1
CSB-EP015079DRM1
CSB-BP015079DRM1
CSB-MP015079DRM1
CSB-EP015079DRM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dryomys nitedula Cytochrome b (MT-CYB), partial CSB-YP015075DMS
CSB-EP015075DMS
CSB-BP015075DMS
CSB-MP015075DMS
CSB-EP015075DMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sistrurus miliarius NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079SZQ1
CSB-EP015079SZQ1
CSB-BP015079SZQ1
CSB-MP015079SZQ1
CSB-EP015079SZQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phocoenoides phocoena Cytochrome b (MT-CYB), partial CSB-YP015075EUS
CSB-EP015075EUS
CSB-BP015075EUS
CSB-MP015075EUS
CSB-EP015075EUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Casuarius bennetti Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073DQL1
CSB-EP015073DQL1
CSB-BP015073DQL1
CSB-MP015073DQL1
CSB-EP015073DQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ambrosia artemisiifolia Non-specific lipid-transfer protein CSB-YP020856BYC
CSB-EP020856BYC
CSB-BP020856BYC
CSB-MP020856BYC
CSB-EP020856BYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Eukaryotic translation initiation factor 3 subunit F (TIF3F1) CSB-YP007535DOA
CSB-EP007535DOA
CSB-BP007535DOA
CSB-MP007535DOA
CSB-EP007535DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vicia faba FACT complex subunit SSRP1 (SSRP1), partial CSB-YP022721VFJ
CSB-EP022721VFJ
CSB-BP022721VFJ
CSB-MP022721VFJ
CSB-EP022721VFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica campestris GTP-binding protein SAR1A (SAR1A) CSB-YP020702BWB
CSB-EP020702BWB
CSB-BP020702BWB
CSB-MP020702BWB
CSB-EP020702BWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uricase (At2g26230) CSB-YP025648DOA
CSB-EP025648DOA
CSB-BP025648DOA
CSB-MP025648DOA
CSB-EP025648DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Signal recognition particle 9 kDa protein (SRP9) CSB-YP022680ZAX
CSB-EP022680ZAX
CSB-BP022680ZAX
CSB-MP022680ZAX
CSB-EP022680ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alnus glutinosa Glutamine synthetase (GLN1) CSB-YP009553AZS
CSB-EP009553AZS
CSB-BP009553AZS
CSB-MP009553AZS
CSB-EP009553AZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solidago canadensis var. scabra Superoxide dismutase [Cu-Zn] (SODCC) CSB-YP022397FIJ
CSB-EP022397FIJ
CSB-BP022397FIJ
CSB-MP022397FIJ
CSB-EP022397FIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium acetobutylicum ATP synthase subunit b (atpF), partial CSB-YP002358DUB1
CSB-EP002358DUB1
CSB-BP002358DUB1
CSB-MP002358DUB1
CSB-EP002358DUB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cystathionine gamma-lyase (mccB) CSB-YP006160BRJ
CSB-EP006160BRJ
CSB-BP006160BRJ
CSB-MP006160BRJ
CSB-EP006160BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconobacter oxydans Inorganic pyrophosphatase (ppa) CSB-YP018410GGU
CSB-EP018410GGU
CSB-BP018410GGU
CSB-MP018410GGU
CSB-EP018410GGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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