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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mesocricetus auratus Class E basic helix-loop-helix protein 22 (BHLHE22) CSB-YP002689MRG
CSB-EP002689MRG
CSB-BP002689MRG
CSB-MP002689MRG
CSB-EP002689MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Presenilin-2 (psen2), partial CSB-YP018847XBE
CSB-EP018847XBE
CSB-BP018847XBE
CSB-MP018847XBE
CSB-EP018847XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eudromia elegans Alpha-crystallin B chain (CRYAB) CSB-YP006008EPA
CSB-EP006008EPA
CSB-BP006008EPA
CSB-MP006008EPA
CSB-EP006008EPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oreochromis niloticus 40S ribosomal protein S12 (rps12) CSB-YP020367OET
CSB-EP020367OET
CSB-BP020367OET
CSB-MP020367OET
CSB-EP020367OET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gadus morhua Hemoglobin subunit beta (hbb) CSB-YP010150GER
CSB-EP010150GER
CSB-BP010150GER
CSB-MP010150GER
CSB-EP010150GER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss DNA-binding protein Ikaros (ikzf1), partial CSB-YP011575OEI
CSB-EP011575OEI
CSB-BP011575OEI
CSB-MP011575OEI
CSB-EP011575OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Indian hedgehog protein (ihh) CSB-YP011569DIK
CSB-EP011569DIK
CSB-BP011569DIK
CSB-MP011569DIK
CSB-EP011569DIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pleurodeles waltl Protein Wnt-5a (WNT5A) CSB-YP026138EWZ
CSB-EP026138EWZ
CSB-BP026138EWZ
CSB-MP026138EWZ
CSB-EP026138EWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphyraena idiastes L-lactate dehydrogenase A chain (ldha) CSB-YP012832FKF
CSB-EP012832FKF
CSB-BP012832FKF
CSB-MP012832FKF
CSB-EP012832FKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L15 (rpl15) CSB-YP020144DOZ
CSB-EP020144DOZ
CSB-BP020144DOZ
CSB-MP020144DOZ
CSB-EP020144DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Acetyl-coenzyme A synthetase (ACS-1), partial CSB-YP001203DWP
CSB-EP001203DWP
CSB-BP001203DWP
CSB-MP001203DWP
CSB-EP001203DWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mitochondrial import inner membrane translocase subunit tim50 (tim50), partial CSB-YP023556SXV1
CSB-EP023556SXV1
CSB-BP023556SXV1
CSB-MP023556SXV1
CSB-EP023556SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Zinc finger protein zpr1 (zpr1) CSB-YP026617SXV
CSB-EP026617SXV
CSB-BP026617SXV
CSB-MP026617SXV
CSB-EP026617SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe V-type proton ATPase subunit d (vma6), partial CSB-YP002401SXV
CSB-EP002401SXV
CSB-BP002401SXV
CSB-MP002401SXV
CSB-EP002401SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Translocation protein sec62 (sec62), partial CSB-YP020960SXV1
CSB-EP020960SXV1
CSB-BP020960SXV1
CSB-MP020960SXV1
CSB-EP020960SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable rRNA-processing protein ebp2 (ebp2) CSB-YP007362SXV
CSB-EP007362SXV
CSB-BP007362SXV
CSB-MP007362SXV
CSB-EP007362SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe U3 small nucleolar ribonucleoprotein protein imp4 (imp4) CSB-YP011693SXV
CSB-EP011693SXV
CSB-BP011693SXV
CSB-MP011693SXV
CSB-EP011693SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Vacuolar protein sorting-associated protein 28 homolog (vps28) CSB-YP025900SXV
CSB-EP025900SXV
CSB-BP025900SXV
CSB-MP025900SXV
CSB-EP025900SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe COP9 signalosome complex subunit 4 (csn4) CSB-YP005791SXV
CSB-EP005791SXV
CSB-BP005791SXV
CSB-MP005791SXV
CSB-EP005791SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Transcription elongation factor spt5 (spt5), partial CSB-YP022959SXV
CSB-EP022959SXV
CSB-BP022959SXV
CSB-MP022959SXV
CSB-EP022959SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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