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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Gamma-glutamyl hydrolase 2 (GGH2) CSB-YP009389DOA
CSB-EP009389DOA
CSB-BP009389DOA
CSB-MP009389DOA
CSB-EP009389DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DNA mismatch repair protein Msh3 (MSH3), partial CSB-YP015029DOA
CSB-EP015029DOA
CSB-BP015029DOA
CSB-MP015029DOA
CSB-EP015029DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carica papaya Superoxide dismutase [Cu-Zn] (SODCC) CSB-YP022397DQH
CSB-EP022397DQH
CSB-BP022397DQH
CSB-MP022397DQH
CSB-EP022397DQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Ribulose-phosphate 3-epimerase (rpe) CSB-YP020097TPR
CSB-EP020097TPR
CSB-BP020097TPR
CSB-MP020097TPR
CSB-EP020097TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus ATP synthase subunit a (atpB), partial CSB-YP015070DNV1
CSB-EP015070DNV1
CSB-BP015070DNV1
CSB-MP015070DNV1
CSB-EP015070DNV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Serine hydroxymethyltransferase (glyA) CSB-YP021274DNV
CSB-EP021274DNV
CSB-BP021274DNV
CSB-MP021274DNV
CSB-EP021274DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Peptide deformylase (def) CSB-YP017707DNV
CSB-EP017707DNV
CSB-BP017707DNV
CSB-MP017707DNV
CSB-EP017707DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Ribonuclease 3 (rnc) CSB-YP019809DNV
CSB-EP019809DNV
CSB-BP019809DNV
CSB-MP019809DNV
CSB-EP019809DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Ribulose-phosphate 3-epimerase (rpe) CSB-YP020097DNV
CSB-EP020097DNV
CSB-BP020097DNV
CSB-MP020097DNV
CSB-EP020097DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus ATP synthase subunit delta (atpH) CSB-YP002355DNV
CSB-EP002355DNV
CSB-BP002355DNV
CSB-MP002355DNV
CSB-EP002355DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus ATP synthase subunit beta (atpD) CSB-YP002350DNV
CSB-EP002350DNV
CSB-BP002350DNV
CSB-MP002350DNV
CSB-EP002350DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Delta-aminolevulinic acid dehydratase (hemB) CSB-YP001558DNV
CSB-EP001558DNV
CSB-BP001558DNV
CSB-MP001558DNV
CSB-EP001558DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Methionyl-tRNA formyltransferase (fmt) CSB-YP015150DNV
CSB-EP015150DNV
CSB-BP015150DNV
CSB-MP015150DNV
CSB-EP015150DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum GTPase Era (era) CSB-YP007759BVW
CSB-EP007759BVW
CSB-BP007759BVW
CSB-MP007759BVW
CSB-EP007759BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203MVZ
CSB-EP001203MVZ
CSB-BP001203MVZ
CSB-MP001203MVZ
CSB-EP001203MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nuclear factor NF-kappa-B p100 subunit (nfkb2), partial CSB-YP015760XBE
CSB-EP015760XBE
CSB-BP015760XBE
CSB-MP015760XBE
CSB-EP015760XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Zinc finger protein ZIC 1 (zic1) CSB-YP026484XBE
CSB-EP026484XBE
CSB-BP026484XBE
CSB-MP026484XBE
CSB-EP026484XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Neuroblastoma suppressor of tumorigenicity 1 (nbl1) CSB-YP015485XBE
CSB-EP015485XBE
CSB-BP015485XBE
CSB-MP015485XBE
CSB-EP015485XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Thyrotropin subunit beta (tshb) CSB-YP025130SWI
CSB-EP025130SWI
CSB-BP025130SWI
CSB-MP025130SWI
CSB-EP025130SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Numida meleagris Interferon gamma (IFNG) CSB-YP011050NHY
CSB-EP011050NHY
CSB-BP011050NHY
CSB-MP011050NHY
CSB-EP011050NHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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