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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rat Protein kinase C iota type (Prkci) CSB-YP018707RA
CSB-EP018707RA
CSB-BP018707RA
CSB-MP018707RA
CSB-EP018707RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine E3 ubiquitin-protein ligase HACE1 (HACE1), partial CSB-YP010115BO
CSB-EP010115BO
CSB-BP010115BO
CSB-MP010115BO
CSB-EP010115BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Ubiquitin carboxyl-terminal hydrolase 37 (USP37), partial CSB-YP025728PI
CSB-EP025728PI
CSB-BP025728PI
CSB-MP025728PI
CSB-EP025728PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Elongator complex protein 2 (ELP2), partial CSB-YP007626DOA
CSB-EP007626DOA
CSB-BP007626DOA
CSB-MP007626DOA
CSB-EP007626DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Biogenesis of lysosome-related organelles complex 1 subunit 2 (BLOS2) CSB-YP002719DOA
CSB-EP002719DOA
CSB-BP002719DOA
CSB-MP002719DOA
CSB-EP002719DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine ATPase family AAA domain-containing protein 1 (ATAD1) CSB-YP002257BO
CSB-EP002257BO
CSB-BP002257BO
CSB-MP002257BO
CSB-EP002257BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloquadratum walsbyi Adenosylhomocysteinase (ahcY) CSB-YP001474HTZ
CSB-EP001474HTZ
CSB-BP001474HTZ
CSB-MP001474HTZ
CSB-EP001474HTZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Enhancer of rudimentary homolog (new10) CSB-YP007785SXV
CSB-EP007785SXV
CSB-BP007785SXV
CSB-MP007785SXV
CSB-EP007785SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Neurotrypsin (Prss12) CSB-YP018812RA
CSB-EP018812RA
CSB-BP018812RA
CSB-MP018812RA
CSB-EP018812RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Eukaryotic elongation factor 2 kinase (EEF2K) CSB-YP007435HU
CSB-EP007435HU
CSB-BP007435HU
CSB-MP007435HU
CSB-EP007435HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L28 CSB-YP020217TQY
CSB-EP020217TQY
CSB-BP020217TQY
CSB-MP020217TQY
CSB-EP020217TQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S acidic ribosomal protein P2 (rpa-2) CSB-YP020342CXY
CSB-EP020342CXY
CSB-BP020342CXY
CSB-MP020342CXY
CSB-EP020342CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant TATA box-binding protein-associated factor RNA polymerase I subunit B (F23H11.2), partial CSB-YP023084CXY
CSB-EP023084CXY
CSB-BP023084CXY
CSB-MP023084CXY
CSB-EP023084CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein LTV1 homolog (T23D8.3) CSB-YP013231CXY
CSB-EP013231CXY
CSB-BP013231CXY
CSB-MP013231CXY
CSB-EP013231CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant MAP kinase-activating death domain protein (aex-3), partial CSB-YP013309CXY
CSB-EP013309CXY
CSB-BP013309CXY
CSB-MP013309CXY
CSB-EP013309CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio hamadryas Insulin-like growth factor-binding protein complex acid labile subunit (IGFALS), partial CSB-YP011094ERJ
CSB-EP011094ERJ
CSB-BP011094ERJ
CSB-MP011094ERJ
CSB-EP011094ERJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Latimeria chalumnae ATP synthase protein 8 (MT-ATP8), partial CSB-YP015071LNS
CSB-EP015071LNS
CSB-BP015071LNS
CSB-MP015071LNS
CSB-EP015071LNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Latimeria chalumnae NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP015080LNS1
CSB-EP015080LNS1
CSB-BP015080LNS1
CSB-MP015080LNS1
CSB-EP015080LNS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratotherium simum NADH-ubiquinone oxidoreductase chain 2 (MT-ND2), partial CSB-YP015077DRG
CSB-EP015077DRG
CSB-BP015077DRG
CSB-MP015077DRG
CSB-EP015077DRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratotherium simum NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078DRG1
CSB-EP015078DRG1
CSB-BP015078DRG1
CSB-MP015078DRG1
CSB-EP015078DRG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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