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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Schizaphis graminum ATP synthase subunit b (atpF), partial CSB-YP002358BXE1
CSB-EP002358BXE1
CSB-BP002358BXE1
CSB-MP002358BXE1
CSB-EP002358BXE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Cysteine desulfurase (iscS) CSB-YP015769BXE
CSB-EP015769BXE
CSB-BP015769BXE
CSB-MP015769BXE
CSB-EP015769BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Electron transfer flavoprotein subunit alpha (etfA) CSB-YP007844MVZ
CSB-EP007844MVZ
CSB-BP007844MVZ
CSB-MP007844MVZ
CSB-EP007844MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Serine palmitoyltransferase 2 (SPTLC2), partial CSB-YP022640DXU1
CSB-EP022640DXU1
CSB-BP022640DXU1
CSB-MP022640DXU1
CSB-EP022640DXU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cytoplasmic protein NCK2 (Nck2) CSB-YP015531MO
CSB-EP015531MO
CSB-BP015531MO
CSB-MP015531MO
CSB-EP015531MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meriones unguiculatus Macrophage migration inhibitory factor (MIF) CSB-YP013826MRA
CSB-EP013826MRA
CSB-BP013826MRA
CSB-MP013826MRA
CSB-EP013826MRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apodemus agrarius Sex-determining region Y protein (SRY) CSB-YP022699DNP
CSB-EP022699DNP
CSB-BP022699DNP
CSB-MP022699DNP
CSB-EP022699DNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Mediator of RNA polymerase II transcription subunit 22 (med22) CSB-YP013655TKY
CSB-EP013655TKY
CSB-BP013655TKY
CSB-MP013655TKY
CSB-EP013655TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP001658FHX
CSB-EP001658FHX
CSB-BP001658FHX
CSB-MP001658FHX
CSB-EP001658FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Cell division control protein 6 homolog (cdc6) CSB-YP005022FHX
CSB-EP005022FHX
CSB-BP005022FHX
CSB-MP005022FHX
CSB-EP005022FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii DNA primase small subunit (priA) CSB-YP018680FHX
CSB-EP018680FHX
CSB-BP018680FHX
CSB-MP018680FHX
CSB-EP018680FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Amidophosphoribosyltransferase (purF) CSB-YP018427FHX
CSB-EP018427FHX
CSB-BP018427FHX
CSB-MP018427FHX
CSB-EP018427FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Nucleoside diphosphate kinase (ndk) CSB-YP015889FHX
CSB-EP015889FHX
CSB-BP015889FHX
CSB-MP015889FHX
CSB-EP015889FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii FAD synthase (ribL) CSB-YP008710FHX
CSB-EP008710FHX
CSB-BP008710FHX
CSB-MP008710FHX
CSB-EP008710FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Glucose-6-phosphate isomerase (pgiA) CSB-YP009717FHX
CSB-EP009717FHX
CSB-BP009717FHX
CSB-MP009717FHX
CSB-EP009717FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Ribosome biogenesis regulatory protein homolog (SPBC29A3.16) CSB-YP020533SXV
CSB-EP020533SXV
CSB-BP020533SXV
CSB-MP020533SXV
CSB-EP020533SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Elongator complex protein 1 (iki3), partial CSB-YP011571SXV
CSB-EP011571SXV
CSB-BP011571SXV
CSB-MP011571SXV
CSB-EP011571SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA polymerase delta subunit 4 (cdm1) CSB-YP018306SXV
CSB-EP018306SXV
CSB-BP018306SXV
CSB-MP018306SXV
CSB-EP018306SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erysiphe graminis subsp. hordei 40S ribosomal protein S12 (RPS12) CSB-YP020367END
CSB-EP020367END
CSB-BP020367END
CSB-MP020367END
CSB-EP020367END-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Methionine aminopeptidase 2 (fma2) CSB-YP013716SXV
CSB-EP013716SXV
CSB-BP013716SXV
CSB-MP013716SXV
CSB-EP013716SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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