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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Drosophila melanogaster 60S ribosomal protein L10 (RpL10) CSB-YP020106DLU
CSB-BP020106DLU
CSB-MP020106DLU
CSB-EP020106DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L37a (rpl-37a) CSB-YP020267OFO
CSB-EP020267OFO
CSB-BP020267OFO
CSB-MP020267OFO
CSB-EP020267OFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Circadian locomoter output cycles protein kaput (Clk), partial CSB-YP005574DLU
CSB-EP005574DLU
CSB-BP005574DLU
CSB-MP005574DLU
CSB-EP005574DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Encephalitozoon cuniculi 60S ribosomal protein L27a (RPL27A) CSB-YP020214EKH
CSB-EP020214EKH
CSB-BP020214EKH
CSB-MP020214EKH
CSB-EP020214EKH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anas acuta NADH-ubiquinone oxidoreductase chain 2 (MT-ND2), partial CSB-YP015077BYX
CSB-EP015077BYX
CSB-BP015077BYX
CSB-MP015077BYX
CSB-EP015077BYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sarcophyton glaucum NADH-ubiquinone oxidoreductase chain 3 (ND3), partial CSB-YP015078SXJ1
CSB-EP015078SXJ1
CSB-BP015078SXJ1
CSB-MP015078SXJ1
CSB-EP015078SXJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glis glis NADH-ubiquinone oxidoreductase chain 5 (MT-ND5), partial CSB-YP015081GGH
CSB-EP015081GGH
CSB-BP015081GGH
CSB-MP015081GGH
CSB-EP015081GGH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrus pyrifolia UTP--glucose-1-phosphate uridylyltransferase CSB-YP025569FIB
CSB-EP025569FIB
CSB-BP025569FIB
CSB-MP025569FIB
CSB-EP025569FIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphatidylinositol N-acetylglucosaminyltransferase subunit P (At1g61280), partial CSB-YP017979DOA1
CSB-EP017979DOA1
CSB-BP017979DOA1
CSB-MP017979DOA1
CSB-EP017979DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytoplasmic tRNA 2-thiolation protein 1 (NCS6) CSB-YP002426DOA
CSB-EP002426DOA
CSB-BP002426DOA
CSB-MP002426DOA
CSB-EP002426DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea mariana Proteasome subunit beta type-3 (PBC1) CSB-YP018880EVM
CSB-EP018880EVM
CSB-BP018880EVM
CSB-MP018880EVM
CSB-EP018880EVM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesembryanthemum crystallinum Delta-1-pyrroline-5-carboxylate synthase (P5CS), partial CSB-YP001564MRC
CSB-EP001564MRC
CSB-BP001564MRC
CSB-MP001564MRC
CSB-EP001564MRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cicer arietinum 60S ribosomal protein L18 (RPL18) CSB-YP020154DTI
CSB-EP020154DTI
CSB-BP020154DTI
CSB-MP020154DTI
CSB-EP020154DTI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zymomonas mobilis Pyruvate dehydrogenase E1 component subunit alpha (pdhA) CSB-YP017716ZBM
CSB-EP017716ZBM
CSB-BP017716ZBM
CSB-MP017716ZBM
CSB-EP017716ZBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus ATP synthase subunit alpha (atpA), partial CSB-YP002344DNV
CSB-EP002344DNV
CSB-BP002344DNV
CSB-MP002344DNV
CSB-EP002344DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Peptide chain release factor 1 (prfA) CSB-YP015197DNV
CSB-EP015197DNV
CSB-BP015197DNV
CSB-MP015197DNV
CSB-EP015197DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799DNV
CSB-EP014799DNV
CSB-BP014799DNV
CSB-MP014799DNV
CSB-EP014799DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Bile salt export pump (Abcb11), partial CSB-YP001049RA
CSB-EP001049RA
CSB-BP001049RA
CSB-MP001049RA
CSB-EP001049RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meriones unguiculatus Beta-1 adrenergic receptor (ADRB1), partial CSB-YP001391MRA1
CSB-EP001391MRA1
CSB-BP001391MRA1
CSB-MP001391MRA1
CSB-EP001391MRA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Lysine-specific demethylase 6A (Kdm6a), partial CSB-YP025774MO
CSB-EP025774MO
CSB-BP025774MO
CSB-MP025774MO
CSB-EP025774MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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