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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP015150RMZ
CSB-EP015150RMZ
CSB-BP015150RMZ
CSB-MP015150RMZ
CSB-EP015150RMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Translation initiation factor IF-3 (infC) CSB-YP015166RLF
CSB-EP015166RLF
CSB-BP015166RLF
CSB-MP015166RLF
CSB-EP015166RLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate aminotransferase (aspC) CSB-YP009681TNN
CSB-EP009681TNN
CSB-BP009681TNN
CSB-MP009681TNN
CSB-EP009681TNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-glucose 6-dehydrogenase (ugd) CSB-YP025567ENL
CSB-EP025567ENL
CSB-BP025567ENL
CSB-MP025567ENL
CSB-EP025567ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Porphobilinogen deaminase (hemC) CSB-YP010524FLF
CSB-EP010524FLF
CSB-BP010524FLF
CSB-MP010524FLF
CSB-EP010524FLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Ribulose-phosphate 3-epimerase (rpe) CSB-YP020097BRJ
CSB-EP020097BRJ
CSB-BP020097BRJ
CSB-MP020097BRJ
CSB-EP020097BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myodes glareolus Phosphatidylcholine-sterol acyltransferase (LCAT) CSB-YP012783MWQ
CSB-EP012783MWQ
CSB-BP012783MWQ
CSB-MP012783MWQ
CSB-EP012783MWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mus musculus bactrianus Proteasome subunit beta type-9 (Psmb9) CSB-YP018887MUW
CSB-EP018887MUW
CSB-BP018887MUW
CSB-MP018887MUW
CSB-EP018887MUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Isopentenyl-diphosphate Delta-isomerase 1 (IDI1) CSB-YP010994MRG
CSB-EP010994MRG
CSB-BP010994MRG
CSB-MP010994MRG
CSB-EP010994MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus NGFI-A-binding protein 1 (NAB1) CSB-YP015401MRG
CSB-EP015401MRG
CSB-BP015401MRG
CSB-MP015401MRG
CSB-EP015401MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Solute carrier family 22 member 6 (Slc22a6), partial CSB-YP021461RA
CSB-EP021461RA
CSB-BP021461RA
CSB-MP021461RA
CSB-EP021461RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alcelaphine herpesvirus 1 Ribonucleoside-diphosphate reductase large subunit (61), partial CSB-YP020518AZG
CSB-EP020518AZG
CSB-BP020518AZG
CSB-MP020518AZG
CSB-EP020518AZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes Protein Wnt-5b (wnt5b) CSB-YP026139OFH
CSB-EP026139OFH
CSB-BP026139OFH
CSB-MP026139OFH
CSB-EP026139OFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplochromis xenognathus Glucose-6-phosphatase (g6pc), partial CSB-YP009118HUJ1
CSB-EP009118HUJ1
CSB-BP009118HUJ1
CSB-MP009118HUJ1
CSB-EP009118HUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Growth/differentiation factor 8 (mstnb) CSB-YP015057DIL
CSB-EP015057DIL
CSB-BP015057DIL
CSB-MP015057DIL
CSB-EP015057DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liza aurata Cytochrome P450 1A1 (cyp1a1), partial CSB-YP006395LQE
CSB-EP006395LQE
CSB-BP006395LQE
CSB-MP006395LQE
CSB-EP006395LQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Progonadoliberin-2 (gnrh2) CSB-YP009636OEI
CSB-EP009636OEI
CSB-BP009636OEI
CSB-MP009636OEI
CSB-EP009636OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aptenodytes patagonicus Thyroid hormone receptor alpha (THRA) CSB-YP023510DNS
CSB-EP023510DNS
CSB-BP023510DNS
CSB-MP023510DNS
CSB-EP023510DNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Cellular retinoic acid-binding protein 1 (crabp1) CSB-YP005935TKY
CSB-EP005935TKY
CSB-BP005935TKY
CSB-MP005935TKY
CSB-EP005935TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oreochromis mossambicus Deoxyribonuclease-1 (dnase1) CSB-YP007049OES
CSB-EP007049OES
CSB-BP007049OES
CSB-MP007049OES
CSB-EP007049OES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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