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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanothermobacter thermautotrophicus FAD synthase (ribL) CSB-YP008710MSR
CSB-EP008710MSR
CSB-BP008710MSR
CSB-MP008710MSR
CSB-EP008710MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Transcription initiation factor IIB (tfb) CSB-YP010001MSR
CSB-EP010001MSR
CSB-BP010001MSR
CSB-MP010001MSR
CSB-EP010001MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus D-3-phosphoglycerate dehydrogenase (serA), partial CSB-YP017920MSR
CSB-EP017920MSR
CSB-BP017920MSR
CSB-MP017920MSR
CSB-EP017920MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033MSR
CSB-EP018033MSR
CSB-BP018033MSR
CSB-MP018033MSR
CSB-EP018033MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917MSR
CSB-EP022917MSR
CSB-BP022917MSR
CSB-MP022917MSR
CSB-EP022917MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Protein archease (MTH_1598) CSB-YP026349MSR
CSB-EP026349MSR
CSB-BP026349MSR
CSB-MP026349MSR
CSB-EP026349MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus DNA topoisomerase 1 (topA), partial CSB-YP024056MSR
CSB-EP024056MSR
CSB-BP024056MSR
CSB-MP024056MSR
CSB-EP024056MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Adenosylhomocysteinase (ahcY) CSB-YP001474MSR
CSB-EP001474MSR
CSB-BP001474MSR
CSB-MP001474MSR
CSB-EP001474MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Methylated-DNA--protein-cysteine methyltransferase (ogt) CSB-YP013788DOC
CSB-EP013788DOC
CSB-BP013788DOC
CSB-MP013788DOC
CSB-EP013788DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824DOC
CSB-EP005824DOC
CSB-BP005824DOC
CSB-MP005824DOC
CSB-EP005824DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus tRNA pseudouridine synthase A (truA) CSB-YP019086DOC
CSB-EP019086DOC
CSB-BP019086DOC
CSB-MP019086DOC
CSB-EP019086DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP001658DOC
CSB-EP001658DOC
CSB-BP001658DOC
CSB-MP001658DOC
CSB-EP001658DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Protein archease (AF_1495) CSB-YP026349DOC
CSB-EP026349DOC
CSB-BP026349DOC
CSB-MP026349DOC
CSB-EP026349DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allochromatium vinosum Cytochrome c1 (petC), partial CSB-YP006327AZP1
CSB-EP006327AZP1
CSB-BP006327AZP1
CSB-MP006327AZP1
CSB-EP006327AZP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Tu (tuf) CSB-YP025342BXC
CSB-EP025342BXC
CSB-BP025342BXC
CSB-MP025342BXC
CSB-EP025342BXC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis tRNA dimethylallyltransferase (miaA) CSB-YP024529BRJ
CSB-EP024529BRJ
CSB-BP024529BRJ
CSB-MP024529BRJ
CSB-EP024529BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine cleavage system H protein (gcvH) CSB-YP009335MVN
CSB-EP009335MVN
CSB-BP009335MVN
CSB-MP009335MVN
CSB-EP009335MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionine synthase (metH), partial CSB-YP015196MVZ
CSB-EP015196MVZ
CSB-BP015196MVZ
CSB-MP015196MVZ
CSB-EP015196MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP015150RMQ
CSB-EP015150RMQ
CSB-BP015150RMQ
CSB-MP015150RMQ
CSB-EP015150RMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP015150RMV
CSB-EP015150RMV
CSB-BP015150RMV
CSB-MP015150RMV
CSB-EP015150RMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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