Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant DNA topoisomerase 1 (topA), partial CSB-YP024056MVN
CSB-EP024056MVN
CSB-BP024056MVN
CSB-MP024056MVN
CSB-EP024056MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor tRNA dimethylallyltransferase (miaA) CSB-YP024529FOB
CSB-EP024529FOB
CSB-BP024529FOB
CSB-MP024529FOB
CSB-EP024529FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Morone saxatilis Progonadoliberin-2 (gnrh2) CSB-YP009636MUL
CSB-EP009636MUL
CSB-BP009636MUL
CSB-MP009636MUL
CSB-EP009636MUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Guanine nucleotide-binding protein subunit alpha-14 (gna14) CSB-YP009586XBE
CSB-EP009586XBE
CSB-BP009586XBE
CSB-MP009586XBE
CSB-EP009586XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Superoxide dismutase [Cu-Zn] (sod1) CSB-YP022397DIL
CSB-BP022397DIL
CSB-MP022397DIL
CSB-EP022397DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Squalus acanthias Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1), partial CSB-YP012087FKW1
CSB-EP012087FKW1
CSB-BP012087FKW1
CSB-MP012087FKW1
CSB-EP012087FKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum Triosephosphate isomerase (tpiA) CSB-YP024102DRA
CSB-EP024102DRA
CSB-BP024102DRA
CSB-MP024102DRA
CSB-EP024102DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe E3 ubiquitin-protein ligase listerin (SPBC21D10.09c), partial CSB-YP026646SXV
CSB-EP026646SXV
CSB-BP026646SXV
CSB-MP026646SXV
CSB-EP026646SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Cytochrome b-c1 complex subunit 7 (qcr7) CSB-YP025664SXV
CSB-EP025664SXV
CSB-BP025664SXV
CSB-MP025664SXV
CSB-EP025664SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa DNA-directed RNA polymerase I subunit RPA2 (acr-2), partial CSB-YP018323NHA
CSB-EP018323NHA
CSB-BP018323NHA
CSB-MP018323NHA
CSB-EP018323NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mitochondrial GTPase 1 (mtg1), partial CSB-YP015152SXV
CSB-EP015152SXV
CSB-BP015152SXV
CSB-MP015152SXV
CSB-EP015152SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA-directed RNA polymerase III subunit rpc4 (rpc53) CSB-YP018346SXV
CSB-EP018346SXV
CSB-BP018346SXV
CSB-MP018346SXV
CSB-EP018346SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DNA-directed RNA polymerase III subunit RPC9 (CRCP) CSB-YP005945HU
CSB-EP005945HU
CSB-BP005945HU
CSB-MP005945HU
CSB-EP005945HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytoplasmic tRNA 2-thiolation protein 1 (tut-1) CSB-YP002426CXY
CSB-EP002426CXY
CSB-BP002426CXY
CSB-MP002426CXY
CSB-EP002426CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera 40S ribosomal protein S8 (RpS8) CSB-YP020478DNK
CSB-EP020478DNK
CSB-BP020478DNK
CSB-MP020478DNK
CSB-EP020478DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 40S ribosomal protein S21 (RpS21) CSB-YP020399DLU
CSB-EP020399DLU
CSB-BP020399DLU
CSB-MP020399DLU
CSB-EP020399DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracentrotus lividus Homeobox protein orthopedia (OTP) CSB-YP017279ERM
CSB-EP017279ERM
CSB-BP017279ERM
CSB-MP017279ERM
CSB-EP017279ERM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Inorganic pyrophosphatase (Nurf-38) CSB-YP018410DLU
CSB-EP018410DLU
CSB-BP018410DLU
CSB-MP018410DLU
CSB-EP018410DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Galactoside 2-alpha-L-fucosyltransferase 2 (FUT2), partial CSB-YP009076EXP1
CSB-EP009076EXP1
CSB-BP009076EXP1
CSB-MP009076EXP1
CSB-EP009076EXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monodelphis domestica Cyclin-dependent kinase inhibitor 2A (CDKN2A) CSB-YP005089MUE
CSB-EP005089MUE
CSB-BP005089MUE
CSB-MP005089MUE
CSB-EP005089MUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>